Evidence map›Paper›PMID 30940443›Full record

ReviewChemistry and physics of lipids2019

Single-particle cryo-EM studies of transmembrane proteins in SMA copolymer nanodiscs.

Chang Sun, Robert B Gennis

Open access · greenAbstract readReview
In one paragraph

Review in Chemistry and physics of lipids, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 40 citations in OpenAlex.

  1. Article
  2. The Evolution of Lipids from Solvents to Substrates.Annual review of biophysics · 2026
    Review
  3. Review
  4. Review
  5. Amphiphilic Copolymers and Their Role in the Study of Membrane Proteins.The journal of physical chemistry letters · 2025
    Review
  6. Article
  7. Article
  8. HisScientific reports · 2023
    Article
  9. Review
  10. Effect of Cholesterol on the Structure and Composition of Glyco-DIBMA Lipid Particles.Langmuir : the ACS journal of surfaces and colloids · 2023
    Article
  11. Review
  12. Review
  13. Review
  14. Article
  15. Review
  16. Article
  17. Review
  18. Rapid preparation of nanodiscs for biophysical studies.Archives of biochemistry and biophysics · 2021
    Article
  19. Cryo-EM structures ofProceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  20. Solid-State NMR Study to Probe the Effects of Divalent Metal Ions (CaLangmuir : the ACS journal of surfaces and colloids · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Chang SunDepartment of Biochemistry, University of Illinois, 600 S. Mathews Street, Urbana, IL 61801, United States. Electronic address: sun.changing@gmail.com.
Robert B GennisDepartment of Biochemistry, University of Illinois, 600 S. Mathews Street, Urbana, IL 61801, United States.
University of Illinois Urbana-Champaign · US

Funding

PROTEIN/LIPID INTERACTIONSR01HL016101 · NHLBI · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI GENNIS, ROBERT B · 1985 to 2015
$6.8M
PROTEIN/LIPID INTERACTIONSR37HL016101 · NHLBI · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI GENNIS, ROBERT B. · 1993 to 2002
$1.5M
NHLBI NIH HHS R01 HL016101NHLBI NIH HHS R37 HL016101
6 · The paper itself

Abstract

Styrene-maleic acid (SMA) copolymers can extract membrane proteins from native membranes along with lipids as nanodiscs. Preparation with SMA is fast, cost-effective, and captures the native protein-lipid interactions. On the other hand, cryo-EM has become increasingly successful and efficient for structural determinations of membrane proteins, with biochemical sample preparation often the bottleneck. Three recent cryo-EM studies on the efflux transporter AcrB and the alternative complex III: cyt c oxidase supercomplex have demonstrated the potential of SMA nanodisc samples to yield high-resolution structure information of membrane proteins.

Indexed as

Cryoelectron MicroscopyMaleatesModels, MolecularNanoparticlesParticle SizePolymersStyreneSurface PropertiesMaleatesmaleic acidPolymersStyreneAlternative complex IIICryo-EMLipid-protein interactionMembrane proteinMultidrug transporter AcrBNanodiscSMAStyrene-maleic acid copolymerTriacylated cysteine

Identifiers

PMID30940443
PMCPMC6500755
OpenAlexW2930585621

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.