ArticleParticle and fibre toxicology2019
In vitro exposure of a 3D-tetraculture representative for the alveolar barrier at the air-liquid interface to silver particles and nanowires.
Article in Particle and fibre toxicology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 51 citations in OpenAlex.
- A human relevant in vitro alveolar epithelial barrier model to assess inhaled pollutant hazard.Scientific reports · 2025Article
- Review
- Multi-array Technology for Secreted Cytokine Profiling in a Nano-aerosol Exposed 3D Human Alveolar Cell Model.Methods in molecular biology (Clifton, N.J.) · 2025Article
- New approach methodologies to enhance human health risk assessment of immunotoxic properties of chemicals - a PARC (Partnership for the Assessment of Risk from Chemicals) project.Frontiers in toxicology · 2024Review
- Transcriptomic Profiling the Effects of Airway Exposure of Zinc Oxide and Silver Nanoparticles in Mouse Lungs.International journal of molecular sciences · 2023Article
- Correlative High-Resolution Imaging of Iron Uptake in Lung Macrophages.Analytical chemistry · 2022Article
- Advanced Respiratory Models for Hazard Assessment of Nanomaterials-Performance of Mono-, Co- and Tricultures.Nanomaterials (Basel, Switzerland) · 2022Article
- Lung Models to Evaluate Silver Nanoparticles' Toxicity and Their Impact on Human Health.Nanomaterials (Basel, Switzerland) · 2022Review
- Comparison of Metal-Based Nanoparticles and Nanowires: Solubility, Reactivity, Bioavailability and Cellular Toxicity.Nanomaterials (Basel, Switzerland) · 2021Article
- In Vitro and In Vivo Models to Assess the Immune-Related Effects of Nanomaterials.International journal of environmental research and public health · 2021Review
- Silver Nanoparticles and Their Antibacterial Applications.International journal of molecular sciences · 2021Review
- Optimization of an air-liquid interfaceJournal of aerosol science · 2021Article
- Pro-inflammatory effects of crystalline- and nano-sized non-crystalline silica particles in a 3D alveolar model.Particle and fibre toxicology · 2020Article
- Mixture Effects of Diesel Exhaust and Metal Oxide Nanoparticles in Human Lung A549 Cells.Nanomaterials (Basel, Switzerland) · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe present study aimed to evaluate the potential differences in the biological effects of two types of spherical silver particles of 20 and 200 nm (Ag20 and Ag200), and of PVP-coated silver nanowires (AgNWs) with a diameter of 50 nm and length up to 50 μm, using a complex 3D model representative for the alveolar barrier cultured at air-liquid interface (ALI). The alveolar model was exposed to 0.05, 0.5 and 5 μg/cm
resultsSignificant cytotoxic effect was observed for all three types of AgNMs at the highest tested doses. The increased mRNA levels of the pro-apoptotic gene CASP7 suggests that apoptosis may occur after exposure to AgNWs. All three types of AgNMs increased the mRNA level of the anti-oxidant enzyme HMOX-1 and of the metal-binding anti-oxidant metallothioneins (MTs), with AgNWs being the most potent inducer. Even though all types of AgNMs induced the nuclear translocation of NF-kB, only AgNWs increased the mRNA level of pro-inflammatory mediators. The pro-inflammatory response elicited by AgNWs was further confirmed by the increased secretion of the 10 evaluated interleukins.
conclusionIn the current study, we demonstrated that the direct exposure of a complex tetra-culture alveolar model to different types of AgNMs at ALI induces shape- and size-specific biological responses. From the three AgNMs tested, AgNWs were the most potent in inducing biological alterations. Starting from 50 ng/cm
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.