Evidence map›Paper›PMID 30940138›Full record

ReviewMicrobial cell factories2019

CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications.

Peng Cai, Jiaoqi Gao, Yongjin Zhou

Abstract readReview
In one paragraph

Review in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.

0numbers the graph read from it
0cells of the map it votes in
66citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

66 citing papers in PubMed.

  1. Article
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  14. A versatile genetic toolkit for engineeringFrontiers in bioengineering and biotechnology · 2025
    Article
  15. Review
  16. New developments in biotechnology applied to microorganisms.EFSA journal. European Food Safety Authority · 2024
    Article
  17. Review
  18. Review
  19. Article
  20. Review

6 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Peng CaiDivision of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, People's Republic of China.
Jiaoqi GaoDivision of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, People's Republic of China.
Yongjin ZhouDivision of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, People's Republic of China. zhouyongjin@dicp.ac.cn.ORCID http://orcid.org/0000-0002-2369-3079

Funding

DMTO research grant DICP DMTO201701National Natural Science Foundation of China 31700082
6 · The paper itself

Abstract

Non-conventional yeasts are playing important roles as cell factories for bioproduction of biofuels, food additives and proteins with outstanding natural characteristics. However, the precise genome editing is challenging in non-conventional yeasts due to lack of efficient genetic tools. In the past few years, CRISPR-based genome editing worked as a revolutionary tool for genetic engineering and showed great advantages in cellular metabolic engineering. Here, we review the current advances and barriers of CRISPR-Cas9 for genome editing in non-conventional yeasts and propose the possible solutions in enhancing its efficiency for precise genetic engineering.

Indexed as

Clustered Regularly Interspaced Short Palindromic RepeatsGenome, FungalBiotechnologyCRISPR-Cas SystemsGene EditingSaccharomyces cerevisiaeCRISPR–Cas9Genome editingGuide RNAHomologous recombinationNon-conventional yeastsNon-homologous end joining

Identifiers

PMID30940138
PMCPMC6444819

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.