Evidence map›Paper›PMID 30939033›Full record

ReviewAnnual review of analytical chemistry (Palo Alto, Calif.)2019

Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing.

Dong Jin Shin, Nadya Andini, Kuangwen Hsieh, Samuel Yang, Tza-Huei Wang

Open access · greenAbstract readReview
In one paragraph

Review in Annual review of analytical chemistry (Palo Alto, Calif.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 1 pooled it
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.

  1. Pooled it
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  12. Culture and amplification-free nanopore sequencing for rapid detection of pathogens and antimicrobial resistance genes from urine.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Dong Jin ShinDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA; email: thwang@jhu.edu.
Nadya AndiniDepartment of Emergency Medicine, Stanford University, Stanford, California 94305, USA; email: syang5@stanford.edu.
Kuangwen HsiehDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA; email: thwang@jhu.edu.
Samuel YangDepartment of Emergency Medicine, Stanford University, Stanford, California 94305, USA; email: syang5@stanford.edu.
Tza-Huei WangDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA; email: thwang@jhu.edu.
Johns Hopkins University · USStanford University · US

Funding

TrainingU54EB007958 · NIBIB · JOHNS HOPKINS UNIVERSITY · PI Susan Anne Tuddenham · 2007 to 2026
$34.6M
A Droplet-based single cell platform for pathogen identification and ASTR01AI117032 · NIAID · JOHNS HOPKINS UNIVERSITY · PI LIAO, JOSEPH C, WANG, TZA-HUEI JEFF · 2015 to 2019
$5.7M
Technology development for point-of-care detection and antimicrobial susceptibility testing of Neisseria gonorrhoeaeR01AI138978 · NIAID · JOHNS HOPKINS UNIVERSITY · PI WANG, TZA-HUEI JEFF · 2018 to 2021
$5.1M
A "Culture" Shift: Integrated Bacterial Screening and Antibacterial Susceptibility Test on Microfluidic Digital Array for Bloodstream InfectionsR01AI137272 · NIAID · JOHNS HOPKINS UNIVERSITY · PI WANG, TZA-HUEI JEFF, YANG, SAMUEL · 2018 to 2022
$3.6M
NIAID NIH HHS R01 AI117032NIAID NIH HHS R01 AI137272NIAID NIH HHS R01 AI138978NIBIB NIH HHS U54 EB007958
6 · The paper itself

Abstract

In the face of looming threats from multi-drug resistant microorganisms, there is a growing need for technologies that will enable rapid identification and drug susceptibility profiling of these pathogens in health care settings. In particular, recent progress in microfluidics and nucleic acid amplification is pushing the boundaries of timescale for diagnosing bacterial infections. With a diverse range of techniques and parallel developments in the field of analytical chemistry, an integrative perspective is needed to understand the significance of these developments. This review examines the scope of new developments in assay technologies grouped by key enabling domains of research. First, we examine recent development in nucleic acid amplification assays for rapid identification and drug susceptibility testing in bacterial infections. Next, we examine advances in microfluidics that facilitate acceleration of diagnostic assays via integration and scale. Lastly, recentdevelopments in biosensor technologies are reviewed. We conclude this review with perspectives on the use of emerging concepts to develop paradigm-changing assays.

Indexed as

AnimalsAnti-Bacterial AgentsBacteriaBacterial InfectionsBiosensing TechniquesHumansMicrobial Sensitivity TestsMicrofluidic Analytical TechniquesNucleic Acid Amplification TechniquesPoint-of-Care SystemsTime FactorsAnti-Bacterial Agentsantibiotic susceptibility testinghigh-resolution melting analysismicrofluidicsmolecular diagnosticsPCRpoint-of-carepolymerase chain reaction

Identifiers

PMID30939033
PMCPMC7369001
OpenAlexW2930500531

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.