Evidence map›Paper›PMID 30938819›Full record

ArticleThe Journal of infectious diseases2019

Chemokine Receptor 7 Is Essential for Coxiella burnetii Whole-Cell Vaccine-Induced Cellular Immunity but Dispensable for Vaccine-Mediated Protective Immunity.

Chen Chen, Erin J van Schaik, Anthony E Gregory, Adam Vigil, Phillip L Felgner, Laura R Hendrix, Robert Faris, James E Samuel

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Review
  3. Impact ofFrontiers in veterinary science · 2022
    Article
  4. Review
  5. Soluble antigens derived fromCell reports. Medicine · 2021
    Article
  6. Review
  7. A Novel Marmoset (Frontiers in cellular and infection microbiology · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Chen ChenDepartment of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center, College of Medicine, Bryan.
Erin J van SchaikDepartment of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center, College of Medicine, Bryan.
Anthony E GregoryDepartment of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center, College of Medicine, Bryan.
Adam VigilDepartment of Medicine, Division of Infectious Diseases, University of California, Irvine.
Phillip L FelgnerDepartment of Medicine, Division of Infectious Diseases, University of California, Irvine.
Laura R HendrixDepartment of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center, College of Medicine, Bryan.
Robert FarisDepartment of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center, College of Medicine, Bryan.
James E SamuelDepartment of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center, College of Medicine, Bryan.
Texas A&M Health Science Center · USUniversity of California, Irvine · US

Funding

WRCE Nonhuman Primate CoreU54AI057156 · NIAID · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI WALKER, DAVID H · 2003 to 2013
$102.6M
VACCINE INTERVENTION AGAINST Q FEVERR01AI048829 · NIAID · TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR · PI SAMUEL, JAMES E · 2000 to 2003
$1.1M
NIAID NIH HHS R01 AI048829NIAID NIH HHS U54 AI057156
6 · The paper itself

Abstract

backgroundProtective immunity against Coxiella burnetii infection is conferred by vaccination with virulent (PI-WCV), but not avirulent (PII-WCV) whole-cell inactivated bacterium. The only well-characterized antigenic difference between virulent and avirulent C. burnetii is they have smooth and rough lipopolysaccharide (LPS), respectively.

methodsMice were vaccinated with PI-WCV and PII-WCV. Humoral and cellular responses were evaluated using protein chip microarrays and ELISpots, respectively. Dendritic cell (DC) maturation after stimulation with PI-WVC and PII-WVC was evaluated using flow cytometry. Vaccine-challenge studies were performed to validate the importance of the receptor CCR7.

resultsOther than specific antibody response to PI-LPS, similar antibody profiles were observed but IgG titers were significantly higher after vaccination with PI-WCV. Furthermore, higher frequency of antigen-specific CD4+ T cells was detected in mice immunized with PI-WCV. PI-WCV-stimulated DCs displayed significantly higher levels of CCR7 and migratory ability to secondary lymphoid organs. Challenge-protection studies in wild-type and CCR7-deficient mice confirmed that CCR7 is critical for PI-WCV-induced cellular immunity.

conclusionsPI-WVC stimulates protective immunity to C. burnetii in mice through stimulation of migratory behavior in DCs for protective cellular immunity. Additionally, the humoral immune response to LPS is an important component of protective immunity.

Indexed as

Immunity, CellularAnimalsAntibodies, BacterialAntibody FormationAntigens, BacterialBacterial VaccinesCoxiella burnetiiDendritic CellsFemaleHumansLipopolysaccharidesMiceQ FeverReceptors, ChemokineVaccinationAntibodies, BacterialAntigens, BacterialBacterial VaccinesLipopolysaccharidesReceptors, Chemokinecellular immune responsesCoxiella burnetiihumoral immune responsesIFN gammaNine Mile INine Mile IIprotein arrayQ fever

Identifiers

PMID30938819
PMCPMC6639598
OpenAlexW2930717653

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.