ArticlePlant, cell & environment2019
A high-throughput transient expression system for rice.
Article in Plant, cell & environment, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- CRISPR-Cas systems for enhancing chilling tolerance in rice: recent advances and future prospects.Biologia futura · 2026Review
- Establishment of a protoplast isolation and transient transformation system for tung tree (Vernicia fordii).Plant methods · 2026Article
- Protoplast isolation and transient gene expression in Suaeda aralocaspica, a halophyte with single-cell CPlant methods · 2026Article
- Efficient Agrobacterium-Mediated Transformation of Green Arabidopsis Suspension Cells.Biotechnology journal · 2025Article
- Article
- A transcription factor ensemble orchestrates bundle sheath expression in rice.Nature communications · 2025Article
- Protoplast-Based Regeneration Enables CRISPR/Cas9 Application in Two TemperatePlants (Basel, Switzerland) · 2025Article
- CtMYB1 regulates flavonoid biosynthesis in safflower flower by binding the CAACCA elements.PloS one · 2025Article
- The rice microRNA159-SPOROCYTELESS EAR2 module regulates starch biosynthesis during pollen development and maintains male fertility.The Plant cell · 2024Article
- Article
- Genome-wide characterization and expression profiling of E2F/DP gene family members in response to abiotic stress in tomato (Solanum lycopersicum L.).BMC plant biology · 2024Article
- DGW1, encoding an hnRNP-like RNA binding protein, positively regulates grain size and weight by interacting with GW6 mRNA.Plant biotechnology journal · 2024Article
- Establishment of protoplasts transient expression system in Pinellia ternata (Thunb.) Breit.Biotechnology letters · 2023Article
- NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems.Nature communications · 2023Article
- AnInternational journal of molecular sciences · 2023Article
- Optimization of Isolation and Transformation of Protoplasts fromInternational journal of molecular sciences · 2023Article
- Artificial intelligence-driven systems engineering for next-generation plant-derived biopharmaceuticals.Frontiers in plant science · 2023Review
- Comprehensive Genome-Wide Analysis and Expression Pattern Profiling ofPlants (Basel, Switzerland) · 2022Article
- Comprehensive Genome-Wide Analysis and Expression Pattern Profiling of theInternational journal of molecular sciences · 2022Article
- DNA Free CRISPR/DCAS9 Based Transcriptional Activation System forPlants (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Rice is an important global crop and represents a vital source of calories for many food insecure regions. Efforts to improve this crop by improving yield, nutritional content, stress tolerance, or resilience to climate change are certain to include biotechnological approaches, which rely on the expression of transgenes in planta. The throughput and cost of currently available transgenic expression systems is frequently incompatible with modern, high-throughput molecular cloning methods. Here, we present a protocol for isolating high yields of green rice protoplasts and for PEG-mediated transformation of isolated protoplasts. Factors affecting transformation efficiency were investigated, and the resulting protocol is fast, cheap, robust, high-throughput, and does not require specialist equipment. When coupled to a high-throughput modular cloning system such as Golden Gate, this transient expression system provides a valuable resource to help break the "design-build-test" bottleneck by permitting the rapid screening of large numbers of transgenic expression cassettes prior to stable plant transformation. We used this system to rapidly assess the expression level, subcellular localisation, and protein aggregation pattern of nine single-gene expression cassettes, which represent the essential component parts of the β-cyanobacterial carboxysome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.