ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2019
Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
- Evolution of a central dopamine circuit underlies adaptation of a light-evoked sensorimotor response in the blind cavefish.Science advances · 2026Article
- A comparative brain atlas in the Mexican cavefish identifies widespread changes in cellular composition and gene expression.bioRxiv : the preprint server for biology · 2026Article
- Host evolution shapes gut microbiome composition inEcology and evolution · 2024Article
- Sensing in the dark: Constructive evolution of the lateral line system in blind populations ofEcology and evolution · 2024Review
- Changes in local interaction rules during ontogeny underlie the evolution of collective behavior.iScience · 2023Article
- Trait Loss in Evolution: What Cavefish Have Taught Us about Mechanisms Underlying Eye Regression.Integrative and comparative biology · 2023Review
- The cavefish Astyanax mexicanus.Nature methods · 2023Article
- Next-generation plasmids for transgenesis in zebrafish and beyond.Development (Cambridge, England) · 2023Article
- Establishment, Long-Term Maintenance, and Characterization of Primary Liver Cells from Astyanax mexicanus.Current protocols · 2023Article
- Novel Husbandry Practices Result in Rapid Rates of Growth and Sexual Maturation Without Impacting Adult Behavior in the Blind Mexican Cavefish.Zebrafish · 2023Article
- Changes in local interaction rules during ontogeny underlie the evolution of collective behavior.bioRxiv : the preprint server for biology · 2023Article
- Natural selection versus neutral mutation in the evolution of subterranean life: A false dichotomy?Frontiers in ecology and evolution · 2023Article
- CaveCrawler: an interactive analysis suite for cavefish bioinformatics.G3 (Bethesda, Md.) · 2022Article
- Establishing a model fish for the Neotropical region: The case of the yellowtail tetraFrontiers in genetics · 2022Review
- Kinematic analysis of social interactions deconstructs the evolved loss of schooling behavior in cavefish.PloS one · 2022Article
- Non-REM and REM/paradoxical sleep dynamics across phylogeny.Current opinion in neurobiology · 2021Review
- Eye morphogenesis in the blind Mexican cavefish.Biology open · 2021Article
- A chromosome-level genome of Astyanax mexicanus surface fish for comparing population-specific genetic differences contributing to trait evolution.Nature communications · 2021Article
- A native, highly active Tc1/mariner transposon from zebrafish (ZB) offers an efficient genetic manipulation tool for vertebrates.Nucleic acids research · 2021Article
- Unlocking the Secrets of the Regenerating Fish Heart: Comparing Regenerative Models to Shed Light on Successful Regeneration.Journal of cardiovascular development and disease · 2021Review
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundAstyanax mexicanus is a well-established fish model system for evolutionary and developmental biology research. These fish exist as surface forms that inhabit rivers and 30 different populations of cavefish. Despite important progress in the deployment of new technologies, deep mechanistic insights into the genetic basis of evolution, development, and behavior have been limited by a lack of transgenic lines commonly used in genetic model systems.
resultsHere, we expand the toolkit of transgenesis by characterizing two novel stable transgenic lines that were generated using the highly efficient Tol2 system, commonly used to generate transgenic zebrafish. A stable transgenic line consisting of the zebrafish ubiquitin promoter expresses enhanced green fluorescent protein ubiquitously throughout development in a surface population of Astyanax. To define specific cell-types, a Cntnap2-mCherry construct labels lateral line mechanosensory neurons in zebrafish. Strikingly, both constructs appear to label the predicted cell types, suggesting many genetic tools and defined promoter regions in zebrafish are directly transferrable to cavefish.
conclusionThe lines provide proof-of-principle for the application of Tol2 transgenic technology in A. mexicanus. Expansion on these initial transgenic lines will provide a platform to address broadly important problems in the quest to bridge the genotype-phenotype gap.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.