ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2019
Matrix Topography Regulates Synaptic Transmission at the Neuromuscular Junction.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- 2D Skeletal Muscle Thin Film Actuators Enhance Efficiency of Biohybrid Robots.bioRxiv : the preprint server for biology · 2026Article
- Inter-Organ Crosstalk and Regulatory Mechanisms of Skeletal Muscle-Derived Extracellular Vesicles in Systemic Metabolic Homeostasis.Journal of cellular and molecular medicine · 2025Review
- Geometrically-engineered human motor assembloids-on-a-chip for neuromuscular interaction readout and hypoxia-driven disease modeling.Nature communications · 2025Article
- Leveraging microtopography to pattern multi-oriented muscle actuators.Biomaterials science · 2025Article
- Actuating Extracellular Matrices Decouple the Mechanical and Biochemical Effects of Muscle Contraction on Motor Neurons.Advanced healthcare materials · 2025Article
- Reply to Caruso Bavisotto et al.: Toward exploring the potential of muscle-secreting exosomes in the muscle-brain axis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Fostering tissue engineering and regenerative medicine to treat musculoskeletal disorders in bone and muscle.Bioactive materials · 2024Review
- Cross-Correlation Increases Sampling in Diffusion-Based Super-Resolution Optical Fluctuation Imaging.Chemical & biomedical imaging · 2024Article
- Neuronal innervation regulates the secretion of neurotrophic myokines and exosomes from skeletal muscle.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Spatially Resolving Size Effects on Diffusivity in Nanoporous Extracellular Matrix-like Materials with Fluorescence Correlation Spectroscopy Super-Resolution Optical Fluctuation Imaging.The journal of physical chemistry. B · 2023Article
- Article
- Tailoring conductive inverse opal films with anisotropic elliptical porous patterns for nerve cell orientation.Journal of nanobiotechnology · 2022Article
- Human neuromuscular junction three-dimensional organoid models and the insight in motor disorders.Journal of molecular cell biology · 2022Article
- Engineering skeletal muscle tissues with advanced maturity improves synapse formation with human induced pluripotent stem cell-derived motor neurons.APL bioengineering · 2021Article
- Nanopattern surface improves cultured human myotube maturation.Skeletal muscle · 2021Article
- Synergistic Effect of Cell-Derived Extracellular Matrices and Topography on Osteogenesis of Mesenchymal Stem Cells.ACS applied materials & interfaces · 2020Article
- Large scale and integrated platform for digital mass culture of anchorage dependent cells.Nature communications · 2019Article
- A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction.eLife · 2019Article
- Matrix Topography Regulates Synaptic Transmission at the Neuromuscular Junction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Recreation of a muscle that can be controlled by the nervous system would provide a major breakthrough for treatments of injury and diseases. However, the underlying basis of how neuron-muscle interfaces are formed is still not understood sufficiently. Here, it is hypothesized that substrate topography regulates neural innervation and synaptic transmission by mediating the cross-talk between neurons and muscles. This hypothesis is examined by differentiating neural stem cells on the myotubes, formed on the substrate with controlled groove width. The substrate with the groove width of 1600 nm, a similar size to the myofibril diameter, serves to produce larger and aligned myotubes than the flat substrate. The myotubes formed on the grooved substrate display increases in the acetylcholine receptor expression. Reciprocally, motor neuron progenitor cells differentiated from neural stem cells innervate the larger and aligned myotubes more actively than randomly oriented myotubes. As a consequence, mature and aligned myotubes respond to glutamate (i.e., an excitatory neurotransmitter) and curare (i.e., a neuromuscular antagonist) more rapidly and homogeneously than randomly oriented myotubes. The results of this study will be broadly useful for improving the quality of engineered muscle used in a series of applications including drug screening, regeneration therapies, and biological machinery assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.