ArticleNature biomedical engineering2019
High-throughput, label-free, single-cell photoacoustic microscopy of intratumoral metabolic heterogeneity.
Article in Nature biomedical engineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Deep computational photoacoustic mesoscopy through heterogeneous tissues enabled by scanning compensation and angular-spectrum enhancement.Photoacoustics · 2026Article
- The prognostic value of metabolic heterogeneity parameter coefficient of variation (COV) of baselineBMC cancer · 2026Article
- Photoacoustic Microscopy for Multiscale Biological System Visualization and Clinical Translation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- The puppet master in the breast cancer "microecological community": spatial and metabolic regulation of macrophage heterogeneity.Molecular cancer · 2026Review
- Tartrazine-enabled optical clearing for in vivo optical resolution photoacoustic microscopy.Biomedical optics express · 2025Article
- A micro-metabolic rewiring assay for assessing hypoxia-associated cancer metabolic heterogeneity.Bioactive materials · 2025Article
- Hybrid transformer-CNN network-driven optical-scanning undersampling for photoacoustic remote sensing microscopy.Photoacoustics · 2025Article
- Emerging Photoacoustic Imaging Techniques for Peripheral Arterial Disease.Current treatment options in cardiovascular medicine · 2025Review
- Super-Low-Dose Functional and Molecular Photoacoustic Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer.Photoacoustics · 2022Article
- Depth-extended acoustic-resolution photoacoustic microscopy based on a two-stage deep learning network.Biomedical optics express · 2022Article
- Video-rate high-resolution single-pixel nonscanning photoacoustic microscopy.Biomedical optics express · 2022Article
- In vivo three-dimensional multispectral photoacoustic imaging of dual enzyme-driven cyclic cascade reaction for tumor catalytic therapy.Nature communications · 2022Article
- Improving Cancer Immunotherapy: Exploring and Targeting Metabolism in Hypoxia Microenvironment.Frontiers in immunology · 2022Review
- Metabolic therapy and bioenergetic analysis: The missing piece of the puzzle.Molecular metabolism · 2021Review
- High-speed functional photoacoustic microscopy using a water-immersible two-axis torsion-bending scanner.Photoacoustics · 2021Article
- Multi-scale optoacoustic molecular imaging of brain diseases.European journal of nuclear medicine and molecular imaging · 2021Review
- Progress of clinical translation of handheld and semi-handheld photoacoustic imaging.Photoacoustics · 2021Review
- Hypoxia-activated probe for NIR fluorescence and photoacoustic dual-mode tumor imaging.iScience · 2021Article
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Abstract
Intratumoral heterogeneity, which is manifested in almost all of the hallmarks of cancer, including the significantly altered metabolic profiles of cancer cells, represents a challenge to effective cancer therapy. High-throughput measurements of the metabolism of individual cancer cells would allow direct visualization and quantification of intratumoral metabolic heterogeneity, yet the throughputs of current measurement techniques are limited to about 120 cells per hour. Here, we show that single-cell photoacoustic microscopy can reach throughputs of approximately 12,000 cells per hour by trapping single cells with blood in an oxygen-diffusion-limited high-density microwell array and by using photoacoustic imaging to measure the haemoglobin oxygen change (that is, the oxygen consumption rate) in the microwells. We demonstrate the capability of this label-free technique by performing high-throughput single-cell oxygen-consumption-rate measurements of cultured cells and by imaging intratumoral metabolic heterogeneity in specimens from patients with breast cancer. High-throughput single-cell photoacoustic microscopy of oxygen consumption rates should enable the faster characterization of intratumoral metabolic heterogeneity.
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