ArticleProceedings of the National Academy of Sciences of the United States of America2019
Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 80 citations in OpenAlex.
- Automated image analysis for intracellular ice detection in monolayers by freeze-substitution.Npj imaging · 2026Article
- Pressure enhanced dual-solid-surface ultra-rapid cooling improves post-thaw recovery in hepatocytes and precision cut liver slices.Scientific reports · 2026Article
- Thermodynamic Principles of Emerging Cryopreservation Technologies.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Microfluidic Platforms for Cryopreservation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Overcoming ice: cutting-edge materials and advanced strategies for effective cryopreservation of biosample.Journal of nanobiotechnology · 2025Review
- Optimisation of cryopreservation conditions, including storage duration and revival methods, for the viability of human primary cells.BMC molecular and cell biology · 2024Article
- Ice formation and its elimination in cryopreservation of oocytes.Scientific reports · 2024Article
- Parallelized Droplet Vitrification Enables Single-Run Vitrification of the Whole Rat Liver Hepatocyte Yield.bioRxiv : the preprint server for biology · 2024Article
- Ice formation and its elimination in cryopreservation of oocytes.Research square · 2024Article
- Impact of cryoprotectant-free sperm vitrification in pulled-glass capillary on sperm parameters and DNA integrity: A lab trial study.International journal of reproductive biomedicine · 2024Article
- Review
- Conduction-Dominated Cryomesh for Organism Vitrification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- [Droplet freeze-thawing system based on solid surface vitrification and laser rewarming].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2023Article
- Technologies for Vitrification Based Cryopreservation.Bioengineering (Basel, Switzerland) · 2023Review
- Cryopreservation of tissues and organs: present, bottlenecks, and future.Frontiers in veterinary science · 2023Review
- Kinetic vitrification: concepts and perspectives in animal sperm cryopreservation.Animal reproduction · 2023Review
- Charge-Dipole Attraction as a Surface Interaction between Water Droplets Immersed in Organic Phases.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Rapid joule heating improves vitrification based cryopreservation.Nature communications · 2022Article
- Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer.Micromachines · 2022Article
- An Overview of Cryo-Scanning Electron Microscopy Techniques for Plant Imaging.Plants (Basel, Switzerland) · 2022Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryopreservation is widely used to maintain backups of cells as it enables the semipermanent storage of cells. During the freezing process, ice crystals that are generated inside and outside the cells can lethally damage the cells. All conventional cryopreservation methods use at least one cryoprotective agent (CPA) to render water inside and outside the cells vitreous or nanocrystallized (near-vitrification) without forming damaging ice crystals. However, CPAs should ideally be avoided due to their cytotoxicity and potential side effects on the cellular state. Herein, we demonstrate the CPA-free cryopreservation of mammalian cells by ultrarapid cooling using inkjet cell printing, which we named superflash freezing (SFF). The SFF cooling rate, which was estimated by a heat-transfer stimulation, is sufficient to nearly vitrify the cells. The experimental results of Raman spectroscopy measurements, and observations with an ultrahigh-speed video camera support the near-vitrification of the droplets under these conditions. Initially, the practical utility of SFF was demonstrated on mouse fibroblast 3T3 cells, and the results were comparable to conventional CPA-assisted methods. Then, the general viability of this method was confirmed on mouse myoblast C2C12 cells and rat primary mesenchymal stem cells. In their entirety, the thus-obtained results unequivocally demonstrate that CPA-free cell cryopreservation is possible by SFF. Such a CPA-free cryopreservation method should be ideally suited for most cells and circumvent the problems typically associated with the addition of CPAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.