ReviewCold Spring Harbor perspectives in biology2019
Group II Intron RNPs and Reverse Transcriptases: From Retroelements to Research Tools.
Review in Cold Spring Harbor perspectives in biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- An Alternative DNA Endonuclease Activity is Associated with the LINE-1 ORF2-encoded Protein.bioRxiv : the preprint server for biology · 2026Article
- Presence of group II introns in phage genomes.Nucleic acids research · 2025Article
- Computational De Novo Design of Group II Introns Yields Highly Active Ribozymes.Chembiochem : a European journal of chemical biology · 2025Article
- Genome-wide profiling of tRNA modifications by Induro-tRNAseq reveals coordinated changes.Nature communications · 2025Article
- Characterization of the mitochondrial genomes forIMA fungus · 2025Article
- Review
- Comparative Mitogenomics Analysis Revealed Evolutionary Divergence amongMicroorganisms · 2024Article
- Article
- Long sequence insertion via CRISPR/Cas gene-editing with transposase, recombinase, and integrase.Current opinion in biomedical engineering · 2023Article
- Article
- Article
- The mitogenomes ofFrontiers in microbiology · 2023Article
- The first two mitochondrial genomes for the genus Ramaria reveal mitochondrial genome evolution of Ramaria and phylogeny of Basidiomycota.IMA fungus · 2022Article
- Characterization of novel small non-coding RNAs and their modifications in bladder cancer using an updated small RNA-seq workflow.Frontiers in molecular biosciences · 2022Article
- Identification of Group II Intron RmInt1 Binding Sites in a Bacterial Genome.Frontiers in molecular biosciences · 2022Article
- TGIRT-seq Protocol for the Comprehensive Profiling of Coding and Non-coding RNA Biotypes in Cellular, Extracellular Vesicle, and Plasma RNAs.Bio-protocol · 2021Article
- A new RNA-DNA interaction required for integration of group II intron retrotransposons into DNA targets.Nucleic acids research · 2021Article
- Prokaryotic reverse transcriptases: from retroelements to specialized defense systems.FEMS microbiology reviews · 2021Review
- Organellar Introns in Fungi, Algae, and Plants.Cells · 2021Review
- Methylation of rRNA as a host defense against rampant group II intron retrotransposition.Mobile DNA · 2021Article
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Authors and funding
2 authors.
Funding
Abstract
Group II introns, self-splicing retrotransposons, serve as both targets of investigation into their structure, splicing, and retromobility and a source of tools for genome editing and RNA analysis. Here, we describe the first cryo-electron microscopy (cryo-EM) structure determination, at 3.8-4.5 Å, of a group II intron ribozyme complexed with its encoded protein, containing a reverse transcriptase (RT), required for RNA splicing and retromobility. We also describe a method called RIG-seq using a retrotransposon indicator gene for high-throughput integration profiling of group II introns and other retrotransposons. Targetrons, RNA-guided gene targeting agents widely used for bacterial genome engineering, are described next. Finally, we detail thermostable group II intron RTs, which synthesize cDNAs with high accuracy and processivity, for use in various RNA-seq applications and relate their properties to a 3.0-Å crystal structure of the protein poised for reverse transcription. Biological insights from these group II intron revelations are discussed.
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