ArticleThe EMBO journal2019
Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy.
Article in The EMBO journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 74 citations in OpenAlex.
- S-palmitoylation coordinates the trafficking of ATG9A to mediate autophagy initiation.Autophagy · 2025Article
- Membrane receptors cluster phosphatidylserine to activate LC3-associated phagocytosis.Nature cell biology · 2025Article
- NOD1 deficiency promotes inflammation via autophagic degradation of ASK1.Communications biology · 2025Article
- Myotubularin 2 interacts with SEC23A and negatively regulates autophagy at ER exit sites in Arabidopsis.Autophagy · 2025Article
- ZDHHC7-mediatedAutophagy · 2024Article
- The pivotal role of autophagy in the pathogenesis and therapy of medulloblastoma.Future oncology (London, England) · 2024Review
- Biogenesis of omegasomes and autophagosomes in mammalian autophagy.Biochemical Society transactions · 2024Review
- ATG16L1 induces the formation of phagophore-like membrane cups.Nature structural & molecular biology · 2024Article
- C16ORF70/MYTHO promotes healthy aging in C.elegans and prevents cellular senescence in mammals.The Journal of clinical investigation · 2024Article
- Article
- Coiled-coil-mediated dimerization of Atg16 is required for binding to the PROPPIN Atg21.Open biology · 2023Article
- Tethering ATG16L1 or LC3 induces targeted autophagic degradation of protein aggregates and mitochondria.Autophagy · 2023Article
- PI3P-dependent regulation of cell size and autophagy by phosphatidylinositol 5-phosphate 4-kinase.Life science alliance · 2023Article
- Article
- Hepatic COX1 loss leads to impaired autophagic flux and exacerbates nonalcoholic steatohepatitis.Acta pharmaceutica Sinica. B · 2023Article
- STING directly recruits WIPI2 for autophagosome formation during STING-induced autophagy.The EMBO journal · 2023Article
- Apicoplast biogenesis mediated by ATG8 requires the ATG12-ATG5-ATG16L and SNAP29 complexes inAutophagy · 2023Article
- ATG16L1 adopts a dual-binding site mode to interact with WIPI2b in autophagy.Science advances · 2023Article
- Effect of ATG12-ATG5-ATG16L1 autophagy E3-like complex on the ability of LC3/GABARAP proteins to induce vesicle tethering and fusion.Cellular and molecular life sciences : CMLS · 2023Article
- LC3-associated endocytosis and the functions of Rubicon and ATG16L1.Science advances · 2022Review
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
Membrane targeting of autophagy-related complexes is an important step that regulates their activities and prevents their aberrant engagement on non-autophagic membranes. ATG16L1 is a core autophagy protein implicated at distinct phases of autophagosome biogenesis. In this study, we dissected the recruitment of ATG16L1 to the pre-autophagosomal structure (PAS) and showed that it requires sequences within its coiled-coil domain (CCD) dispensable for homodimerisation. Structural and mutational analyses identified conserved residues within the CCD of ATG16L1 that mediate direct binding to phosphoinositides, including phosphatidylinositol 3-phosphate (PI3P). Mutating putative lipid binding residues abrogated the localisation of ATG16L1 to the PAS and inhibited LC3 lipidation. On the other hand, enhancing lipid binding of ATG16L1 by mutating negatively charged residues adjacent to the lipid binding motif also resulted in autophagy inhibition, suggesting that regulated recruitment of ATG16L1 to the PAS is required for its autophagic activity. Overall, our findings indicate that ATG16L1 harbours an intrinsic ability to bind lipids that plays an essential role during LC3 lipidation and autophagosome maturation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.