ArticleBMC veterinary research2019
Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducks.
Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Development and validation of a crystal digital PCR assay for duck Tembusu virus detection and vaccine monitoring.Poultry science · 2026Article
- Pathogenic mechanisms and molecular features of a novel UL2 gene-deficient duck enteritis virus endemic to China.Virulence · 2025Article
- Development of a Multiplex Quantitative Polymerase Chain Reaction Assay for the Detection of Duck Enteritis Virus, Goose Parvovirus, and Muscovy Duck Parvovirus.Animals : an open access journal from MDPI · 2025Article
- Development of a Multiplex Conventional PCR Assay for Concurrent Detection of FAdV-4, FAdV-8b, and FAdV-11.Veterinary sciences · 2025Article
- Establishment of one-step duplex TaqMan real-time PCR for detection of feline coronavirus and panleukopenia virus.Applied microbiology and biotechnology · 2025Article
- ExploringVeterinary sciences · 2025Article
- A snapshot on molecular technologies for diagnosing FAdV infections.Frontiers in veterinary science · 2025Review
- Article
- Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a.Animals : an open access journal from MDPI · 2024Article
- One-Step Multiplex Real-Time Fluorescent Quantitative Reverse Transcription PCR for Simultaneous Detection of Four Waterfowl Viruses.Microorganisms · 2024Article
- PCR in Forensic Science: A Critical Review.Genes · 2024Review
- Simultaneous and rapid detection of avian respiratory diseases of small poultry using multiplex reverse transcription-Polymerase Chain Reaction assay.Poultry science · 2023Article
- Isolation and genomic characterization of one novel goose astrovirus causing acute gosling gout in China.Scientific reports · 2023Article
- Establishment of a triplex TaqMan quantitative real-time PCR assay for simultaneous detection ofFrontiers in microbiology · 2023Article
- Article
- Molecular Epidemiology of Antibiotic Resistance Genes and Virulence Factors in Multidrug-ResistantInternational journal of environmental research and public health · 2022Article
- Duck virus enteritis (duck plague) outbreak in an Australian black swan (Journal of advanced veterinary and animal research · 2021Article
- Establishment of TaqMan-based real-time PCR assay for rapid detection of duck circovirus.3 Biotech · 2021Article
- New Insights into the Biology of the Emerging Tembusu Virus.Pathogens (Basel, Switzerland) · 2021Review
- Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1.Frontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundMajor viruses, including duck-origin avian influenza virus, duck-origin Newcastle disease virus, novel duck parvovirus, duck hepatitis A virus, duck Tembusu virus, fowl adenovirus, and duck enteritis virus, pose great harm to ducks and cause enormous economic losses to duck industry. This study aims to establish a multiplex polymerase chain reaction (m-PCR) method for simultaneous detection of these seven viruses.
resultsSpecific primers were designed and synthesized according to the conserved region of seven viral gene sequences. Then, seven recombinant plasmids, as the positive controls, were reconstructed in this study. Within the study, D-optimal design was adopted to optimize PCR parameters. The optimum parameters for m-PCR were annealing temperature at 57 °C, Mg
conclusionGiven its rapidity, specificity, sensitivity, and convenience, the established m-PCR method is feasible for simultaneous detection of seven duck-infecting viruses and can be applied to clinical diagnosis of viral infection in ducks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.