Evidence map›Paper›PMID 30934749›Full record

ArticleInternational journal of environmental research and public health2019

Metagenomic Profiling of Microbial Pathogens in the Little Bighorn River, Montana.

Steve Hamner, Bonnie L Brown, Nur A Hasan, Michael J Franklin, John Doyle, Margaret J Eggers, Rita R Colwell, Timothy E Ford

Abstract read
In one paragraph

Article in International journal of environmental research and public health, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Genetic Basis of Multiple Antibiotic Resistance of PathogenicInternational journal of molecular sciences · 2025
    Article
  2. Article
  3. Review
  4. Article
  5. Microbial Ecotoxicology-40 Years on.Life (Basel, Switzerland) · 2025
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Our Relationship to Water and Experience of Water Insecurity among Apsáalooke (Crow Indian) People, Montana.International journal of environmental research and public health · 2021
    Article
  17. Article
  18. Emerging priorities for microbial metagenome research.Bioresource technology reports · 2020
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Steve HamnerDepartment of Environmental Health Sciences, School of Public Health & Health Sciences, University of Massachusetts Amherst, Amherst, MA 01003, USA 2 Department of Microbiology, Montana State University, Bozeman, MT 59717, USA. shamner@montana.edu.
Bonnie L BrownDepartment of Biological Sciences, University of New Hampshire, Durham, NH 03824, USA. bonnie.brown@unh.edu.
Nur A HasanCosmosID Inc., 1600 East Gude Drive, Rockville, MD 20850, USA. nur.hasan@cosmosid.com.ORCID 0000-0002-9179-5114
Michael J FranklinDepartment of Microbiology, Montana State University, Bozeman, MT 59717, USA. franklin@montana.edu.
John DoyleCrow Water Quality Project, Crow Agency, Little Big Horn College, MT 59022, USA. doylej@lbhc.edu.
Margaret J EggersDepartment of Microbiology, Montana State University, Bozeman, MT 59717, USA. mari.eggers@montana.edu.ORCID 0000-0002-9179-5114
Rita R ColwellCosmosID Inc., 1600 East Gude Drive, Rockville, MD 20850, USA. rcolwell@umiacs.umd.edu.
Timothy E FordDepartment of Environmental Health Sciences, School of Public Health & Health Sciences, University of Massachusetts Amherst, Amherst, MA 01003, USA 2 Department of Microbiology, Montana State University, Bozeman, MT 59717, USA. teford@umass.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Little Bighorn River is the primary source of water for water treatment plants serving the local Crow Agency population, and has special significance in the spiritual and ceremonial life of the Crow tribe. Unfortunately, the watershed suffers from impaired water quality, with high counts of fecal coliform bacteria routinely measured during run-off events. A metagenomic analysis was carried out to identify potential pathogens in the river water. The Oxford Nanopore MinION platform was used to sequence DNA in near real time to identify both uncultured and a coliform-enriched culture of microbes collected from a popular summer swimming area of the Little Bighorn River. Sequences were analyzed using CosmosID bioinformatics and, in agreement with previous studies, enterohemorrhagic and enteropathogenic

Indexed as

EnterobacteriaceaeEscherichia coliHumansMetagenomicsMontanaRiversSerogroupVirulenceWater Qualitymetagenomicspathogen detectionwaterborne disease

Identifiers

PMID30934749
PMCPMC6479903

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.