ArticleReproduction (Cambridge, England)2019
Persistent effects on bovine granulosa cell transcriptome after resolution of uterine disease.
Article in Reproduction (Cambridge, England), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Protective effects of linoleic acid against E. coli lipopolysaccharide-induced oxidative and nitrosative toxicity in buffalo luteal cell culture.BMC veterinary research · 2026Article
- Dominant follicle-targeted nanocarriers for GH delivery to alleviate premature ovarian insufficiency.Materials today. Bio · 2025Article
- Ovarian Stimulation Altered Uterine Fluid Extracellular Vesicles miRNA Affecting Implantation in Rats.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Exploring the genetic control of sweat gland characteristics in beef cattle for enhanced heat tolerance.Journal of animal science and biotechnology · 2024Article
- Effects of Elective Caesarean Sections in Healthy Near-Term Ewes on Subsequent Reproductive Performance.Animals : an open access journal from MDPI · 2024Article
- Screening of Litter-Size-Associated SNPs inAnimals : an open access journal from MDPI · 2024Article
- Detection of Candidate Genes Associated with Fecundity through Genome-Wide Selection Signatures of Katahdin Ewes.Animals : an open access journal from MDPI · 2023Article
- Effects of lipopolysaccharide on follicular estrogen production and developmental competence in bovine oocytes.Animal reproduction science · 2022Article
- Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells.CABI agriculture and bioscience · 2022Article
- The endometrial transcriptomic response to pregnancy is altered in cows after uterine infection.PloS one · 2022Article
- Article
- Understanding the trade-off between the environment and fertility in cows and ewes.Animal reproduction · 2020Review
- Experimentally Induced Endometritis Impairs the Developmental Capacity of Bovine Oocytes†.Biology of reproduction · 2020Article
- Recent advances and future directions for uterine diseases diagnosis, pathogenesis, and management in dairy cows.Animal reproduction · 2020Review
- Uterine infusion of bacteria alters the transcriptome of bovine oocytes.FASEB bioAdvances · 2020Article
- Uterine infection alters the transcriptome of the bovine reproductive tract three months later.Reproduction (Cambridge, England) · 2020Article
- Lipopolysaccharide and tumor necrosis factor-alpha alter gene expression of oocytes and cumulus cells during bovine in vitro maturation.Molecular reproduction and development · 2019Article
- Inflammation: friend or foe of bovine reproduction?Animal reproduction · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Metritis is associated with reduced fertility in dairy cows, but the mechanisms are unclear because the disease resolves several weeks before insemination. One hypothesis is that metritis causes persistent changes in granulosa cells during follicle development, which might be evident in the transcriptome of granulosa cells from dominant follicles weeks after parturition. To test this hypothesis, we collected the follicular fluid and granulosa cells from dominant follicles 63 days post partum from cows previously diagnosed with metritis, at least 6 weeks after resolution of the disease and from cows not diagnosed with metritis (control cows). Bacterial lipopolysaccharide was detected in follicular fluid, and concentrations were associated with follicular fluid IL-8 and glucose concentrations. Transcriptome analysis using RNAseq revealed 177 differentially expressed genes in granulosa cells collected from cows that had metritis compared with control cows. The most upregulated genes were ITLN1, NCF2, CLRN3, FSIP2 and ANKRD17, and the most downregulated genes were ACSM1, NR4A2, GHITM, CBARP and NR1I3. Pathway analysis indicated that the differentially expressed genes were involved with immune function, cell-cell communication, cell cycle and cellular metabolism. Predicted upstream regulators of the differentially expressed genes included NFκB, IL-21 and lipopolysaccharide, which are associated with infection and immunity. Our data provide evidence for a persistent effect of metritis on the transcriptome of granulosa cells in ovarian follicles after the resolution of disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.