ArticleScientific reports2019
Illumina sequencing of clinical samples for virus detection in a public health laboratory.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- Medical software for precision diagnostics of infection with immunoprofiling and artificial intelligence.Journal of translational medicine · 2026Review
- Multiplex metagenomic sequencing for rapid viral pathogen identification and surveillance in clinical specimens.BMC infectious diseases · 2025Article
- Decreases in chimpanzee respiratory disease signs and enteric viral quantity following implementation of anthroponotic disease prevention protocols at a long-term research site.Biological conservation · 2025Article
- AI-powered analysis of viral metagenomic sequencing data for rapid outbreak investigation and novel pathogen discovery.Frontiers in microbiology · 2025Review
- Improving Bacterial Metagenomic Research through Long-Read Sequencing.Microorganisms · 2024Article
- Wastewater-based epidemiology applied at the building-level reveals distinct virome profiles based on the age of the contributing individuals.Human genomics · 2024Article
- Rapid Real-time Squiggle Classification for Read until using RawMap.Archives of clinical and biomedical research · 2023Article
- Article
- SARS-CoV-2 wastewater monitoring using a novel PCR-based method rapidly captured the Delta-to-Omicron ΒΑ.1 transition patterns in the absence of conventional surveillance evidence.The Science of the total environment · 2022Article
- Female reproduction and viral infection in a long-lived mammal.The Journal of animal ecology · 2022Article
- Strategies for managing marine disease.Ecological applications : a publication of the Ecological Society of America · 2022Article
- Metatranscriptomic Analysis of Plant Viruses in Imported Pear and Kiwifruit Pollen.The plant pathology journal · 2022Article
- Quantitative and ultrasensitive in situ immunoassay technology for SARS-CoV-2 detection in saliva.Science advances · 2022Article
- Viruses associated with ill health in wild chimpanzees.American journal of primatology · 2022Article
- RNA Virus Gene Signatures Detected in Patients With Cardiomyopathy After Chemotherapy; A Pilot Study.Frontiers in cardiovascular medicine · 2022Article
- Monitoring the SARS-CoV-2 pandemic: screening algorithm with single nucleotide polymorphism detection for the rapid identification of established and emerging variants.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2022Article
- Comparing variability in diagnosis of upper respiratory tract infections in patients using syndromic, next generation sequencing, and PCR-based methods.PLOS global public health · 2022Article
- SARS-CoV-2 genomic diversity and the implications for qRT-PCR diagnostics and transmission.Genome research · 2021Article
- Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus.The Journal of general virology · 2021Article
- Quantitative and Ultrasensitive In-situ Immunoassay Technology for SARS-CoV-2 Detection in Saliva.Research square · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput sequencing (HTS) provides the opportunity, once a diagnostic result is obtained, to extract additional information from a virus-containing sample. Hence, it offers advantages over established quantitative amplification technology, such as quantitative PCR, particularly in a public health environment. At this early stage of its clinical application, there have been limited studies comparing HTS performance to that of the more established quantitative PCR technology for direct detection of viruses. In this pilot-scale study, we tested HTS with a range of viruses and sample types routinely encountered in a public health virology laboratory. In comparison with quantitative PCR, our HTS method was able to sensitively (92%) detect all viruses in any sample type with the exception of certain tissues. Moreover, sufficient nucleotide sequence information was obtained to enable genotyping of strains detected, thus providing additional useful epidemiological information. While HTS sensitivity may not yet match that of PCR, the added value through enhanced epidemiological data has considerable potential to enable real-time surveillance of circulating strains so as to facilitate rapid and appropriate response to outbreaks and virus zoonotic spillover events.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.