ArticleScientific data2019
Targeted enrichment by solution-based hybrid capture to identify genetic sequence variants in barley.
Article in Scientific data, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Machine learning to predict genotypes and genotype-environment interaction associated with complex traits for genomic selection.Plant phenomics (Washington, D.C.) · 2026Article
- Correcting errors in PCR-derived libraries for rare allele detection by reconstructing parental and daughter strand information.Communications biology · 2025Article
- Diversity of Gibberellin 2-oxidase genes in the barley genome offers opportunities for genetic improvement.Journal of advanced research · 2024Article
- Single-molecule long-read methylation profiling reveals regional DNA methylation regulated by Elongator Complex Subunit 2 in Arabidopsis roots experiencing spaceflight.Biology direct · 2024Article
- Rare but diverse off-target and somatic mutations found in field and greenhouse grown trees expressing CRISPR/Cas9.Frontiers in bioengineering and biotechnology · 2024Article
- Next-generation fungal identification using target enrichment and Nanopore sequencing.BMC genomics · 2023Article
- New semi-dwarfing alleles with increased coleoptile length by gene editing of gibberellin 3-oxidase 1 using CRISPR-Cas9 in barley (Hordeum vulgare L.).Plant biotechnology journal · 2023Article
- Multi-locus genome-wide association studies reveal novel alleles for flowering time under vernalisation and extended photoperiod in a barley MAGIC population.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Development and application of the Faba_bean_130K targeted next-generation sequencing SNP genotyping platform based on transcriptome sequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Genomic structural equation modelling provides a whole-system approach for the future crop breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Article
- Genome-wide association mapping reveals novel genes associated with coleoptile length in a worldwide collection of barley.BMC plant biology · 2020Article
- Genetic dissection of the interactions between semi-dwarfing genesMolecular breeding : new strategies in plant improvement · 2020Article
- Genome-Wide Association Study and Identification of Candidate Genes for Nitrogen Use Efficiency in Barley (Frontiers in plant science · 2020Article
- CRISPR-Cas9 enrichment and long read sequencing for fine mapping in plants.Plant methods · 2020Article
- Genome-Wide Association Study of Salinity Tolerance During Germination in Barley (Frontiers in plant science · 2020Article
- Gene-set association and epistatic analyses reveal complex gene interaction networks affecting flowering time in a worldwide barley collection.Journal of experimental botany · 2019Article
- Targeted enrichment by solution-based hybrid capture to identify genetic sequence variants in barley.Scientific data · 2019Article
- Truly the best of both worlds: Merging lineage-specific and universal probe kits to maximize phylogenomic inference.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In barley and other cereal crops, phenological diversity drives adaptation to different cultivation areas. Improvement of barley yield and quality traits requires adaptation to specific production areas with introgression of favorable alleles dependent upon precise identification of the underlying genes. Combining targeted sequence capture systems with next-generation sequencing provides an efficient approach to explore target genetic regions at high resolution, and allows rapid discovery of thousands of genetic polymorphisms. Here, we apply a versatile target-capture method to detect genome-wide polymorphisms in 174 flowering time-related genes, chosen based on prior knowledge from barley, rice, and Arabidopsis thaliana. Sequences were generated across a phenologically diverse panel of 895 barley varieties, resulting a high mean depth coverage of ~25x allowing reliable discovery and calling of insertion-deletion (InDel) and single nucleotide polymorphisms (SNPs). Sequences of InDel and SNPs from the targeted enrichment were utilized to develop 67 Kompetitive Allele Specific PCR (KASP) markers for validation. This work provides researchers and breeders a comprehensive molecular toolkit for the selection of phenology-related traits in barley.
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