ArticleMaterials horizons2019
Ice-recrystallization inhibiting polymers protect proteins against freeze-stress and enable glycerol-free cryostorage.
Article in Materials horizons, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
26 citing papers in PubMed, 78 citations in OpenAlex.
- Cryopreservation technology for improving the stability of liposomes and its precise drug monitoring in clinical drug research.Drug delivery · 2026Article
- Impact of single freeze-thaw cycles on human serum proteins: Implications for mass spectrometry biomarker validation.iScience · 2026Article
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- Not only an inhibitor: Trehalose enhances the catalytic action exerted on oxaloacetate by rabbit lactate dehydrogenase.Protein science : a publication of the Protein Society · 2025Article
- PVAylation: precision end-functionalized poly(vinyl alcohol) for site-selective bioconjugation.Chemical science · 2025Article
- Rediscovery of poly(ethylene glycol)s as a cryoprotectant for mesenchymal stem cells.Biomaterials research · 2023Article
- Synthesis of Poly(2-(methylsulfinyl)ethyl methacrylate) via Oxidation of Poly(2-(methylthio)ethyl methacrylate): Evaluation of the Sulfoxide Side Chain on Cryopreservation.ACS polymers Au · 2022Article
- Synthesis of poly(vinyl alcohol) by blue light bismuth oxide photocatalysed RAFT. Evaluation of the impact of freeze/thaw cycling on ice recrystallisation inhibition.Polymer chemistry · 2022Article
- Degradable Polyampholytes from Radical Ring-Opening Copolymerization Enhance Cellular Cryopreservation.ACS macro letters · 2022Article
- Ice-binding proteins and bioinspired synthetic mimics in non-physiological environments.iScience · 2022Review
- Red Blood Cell Cryopreservation with Minimal Post-Thaw Lysis Enabled by a Synergistic Combination of a Cryoprotecting Polyampholyte with DMSO/Trehalose.Biomacromolecules · 2022Article
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- Polymer-Mediated Cryopreservation of Bacteriophages.Biomacromolecules · 2021Article
- Integrated Graphene Oxide with Noble Metal Nanoparticles to Develop High-Sensitivity Fiber Optic Particle Plasmon Resonance (FOPPR) Biosensor for Biomolecules Determination.Nanomaterials (Basel, Switzerland) · 2021Article
- Synergistic Ice Inhibition Effect Enhances Rapid Freezing Cryopreservation with Low Concentration of Cryoprotectants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- The atomistic details of the ice recrystallisation inhibition activity of PVA.Nature communications · 2021Article
- Protection of Alcohol Dehydrogenase against Freeze-Thaw Stress by Ice-Binding Proteins Is Proportional to Their Ice Recrystallization Inhibition Property.Marine drugs · 2020Article
- Ice recrystallisation inhibiting polymers prevent irreversible protein aggregation during solvent-free cryopreservation as additives and as covalent polymer-protein conjugates.European polymer journal · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins are ubiquitous in molecular biotechnology, biotechnology and as therapeutics, but there are significant challenges in their storage and distribution, with freezing often required. This is traditionally achieved by the addition of cryoprotective agents such as glycerol (or trehalose) or covalent modification of mutated proteins with cryoprotectants. Here, ice recrystallization inhibiting polymers, inspired by antifreeze proteins, are used synergistically with poly(ethylene glycol) as an alternative to glycerol. The primary mechanism of action appears to be preventing irreversible aggregation due to ice growth. The polymer formulation is successfully used to cryopreserve a range of important proteins including insulin, Taq DNA polymerase and an IgG antibody. The polymers do not require covalent conjugation, nor modification of the protein and are already used in a wide range of biomedical applications, which will facilitate translation to a range of biologics.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.