ArticleFrontiers in plant science2019
Development of a Sequence-Based Reference Physical Map of Pea (
Article in Frontiers in plant science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Genome-wide association studies of a pea germplasm reveal novel markers and candidate genes implicated in resistance to Fusarium oxysporum f. sp. pisi races 1 and 2.The plant genome · 2026Article
- Genome-wide characterization and expression profiling of FARL (FHY3/FAR1) family genes in Zea mays.Journal, genetic engineering & biotechnology · 2024Article
- Pea Breeding for Resistance to Rhizospheric Pathogens.Plants (Basel, Switzerland) · 2022Review
- Understanding Starch Metabolism in Pea Seeds towards Tailoring Functionality for Value-Added Utilization.International journal of molecular sciences · 2021Review
- Omics resources and omics-enabled approaches for achieving high productivity and improved quality in pea (Pisum sativum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Rooibos (Plants (Basel, Switzerland) · 2020Article
- A reference genome for pea provides insight into legume genome evolution.Nature genetics · 2019Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whole genome profiling (WGP) is a sequence-based physical mapping technology and uses sequence tags generated by next generation sequencing for construction of bacterial artificial chromosome (BAC) contigs of complex genomes. The physical map provides a framework for assembly of genome sequence and information for localization of genes that are difficult to find through positional cloning. To address the challenges of accurate assembly of the pea genome (∼4.2 GB of which approximately 85% is repetitive sequences), we have adopted the WGP technology for assembly of a pea BAC library. Multi-dimensional pooling of 295,680 BAC clones and sequencing the ends of restriction fragments of pooled DNA generated 1,814 million high quality reads, of which 825 million were deconvolutable to 1.11 million unique WGP sequence tags. These WGP tags were used to assemble 220,013 BACs into contigs. Assembly of the BAC clones using the modified Fingerprinted Contigs (FPC) program has resulted in 13,040 contigs, consisting of 213,719 BACs, and 6,294 singleton BACs. The average contig size is 0.33 Mbp and the N
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.