ArticleGreen chemistry : an international journal and green chemistry resource : GC2019
The identification and use of robust transaminases from a domestic drain metagenome.
Article in Green chemistry : an international journal and green chemistry resource : GC, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 67 citations in OpenAlex.
- Harnessing Bacillus inaquosorum AGSP2 for enhancing ω-transaminase production through classical and AI-supported statistical design.Scientific reports · 2026Article
- Transaminase and Norcoclaurine Synthase One-Pot Cascades Towards (1S)-Tetrahydroisoquinolines.Chembiochem : a European journal of chemical biology · 2026Article
- The discovery of new metagenomic urethanases utilising a novel colorimetric assay for applications in the biodegradation of polyurethanes.Green chemistry : an international journal and green chemistry resource : GC · 2025Article
- Stereoselective Chemoenzymatic Cascades for the Synthesis of Densely Functionalized Iminosugars.Journal of the American Chemical Society · 2025Article
- Discovery and biocatalytic characterization of opine dehydrogenases by metagenome mining.Applied microbiology and biotechnology · 2024Article
- The Impact of Metagenomics on Biocatalysis.Angewandte Chemie (International ed. in English) · 2024Review
- A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines.Catalysis science & technology · 2024Article
- Natural transaminase fusions for biocatalysis.RSC advances · 2024Article
- RetroBioCat Database: A Platform for Collaborative Curation and Automated Meta-Analysis of Biocatalysis Data.ACS catalysis · 2023Article
- Novel transaminases from thermophiles: from discovery to application.Microbial biotechnology · 2022Article
- Direct Conversion of Hydrazones to Amines using Transaminases.ChemCatChem · 2021Article
- Discovery of New Carbonyl Reductases Using Functional Metagenomics and Applications in Biocatalysis.Advanced synthesis & catalysis · 2021Article
- Biocatalytic access to betazole using a one-pot multienzymatic system in continuous flow.Green chemistry : an international journal and green chemistry resource : GC · 2021Article
- Screening and characterization of a diverse panel of metagenomic imine reductases for biocatalytic reductive amination.Nature chemistry · 2021Article
- Identification and catalytic properties of new epoxide hydrolases from the genomic data of soil bacteria.Enzyme and microbial technology · 2020Article
- Metagenomic applications in exploration and development of novel enzymes from nature: a review.Journal, genetic engineering & biotechnology · 2020Review
- Transaminases for industrial biocatalysis: novel enzyme discovery.Applied microbiology and biotechnology · 2020Review
- The Hitchhiker's guide to biocatalysis: recent advances in the use of enzymes in organic synthesis.Chemical science · 2020Review
- Metagenomic ene-reductases for the bioreduction of sterically challenging enones.RSC advances · 2019Article
- Aminopolyols from Carbohydrates: Amination of Sugars and Sugar-Derived Tetrahydrofurans with Transaminases.Angewandte Chemie (International ed. in English) · 2019Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transaminases remain one of the most promising biocatalysts for use in chiral amine synthesis, however their industrial implementation has been hampered by their general instability towards, for example, high amine donor concentrations and organic solvent content. Herein we describe the identification, cloning and screening of 29 novel transaminases from a household drain metagenome. The most promising enzymes were fully characterised and the effects of pH, temperature, amine donor concentration and co-solvent determined. Several enzymes demonstrated good substrate tolerance as well as an unprecedented robustness for a wild-type transaminase. One enzyme in particular readily accepted IPA as an amine donor giving the same conversion with 2-50 equivalents, as well as being tolerant to a number of co-solvents, and operational in up to 50% DMSO - a characteristic as yet unobserved in a wild-type transaminase. This work highlights the value of using metagenomics for biocatalyst discovery from niche environments, and here has led to the identification of one of the most robust native transaminases described to date, with respect to IPA and DMSO tolerance.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.