Evidence map›Paper›PMID 30929607›Full record

ArticleRNA biology2019

Systematic evaluation of the microRNAome through miR-CATCHv2.0 identifies positive and negative regulators of

Andrea Marranci, Romina D'Aurizio, Sebastian Vencken, Serena Mero, Elena Guzzolino, Milena Rizzo, Letizia Pitto, Marco Pellegrini, Giovanna Chiorino, Catherine M Greene and 1 more

Open access · bronzeAbstract read
In one paragraph

Article in RNA biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Andrea Marrancia Institute of Clinical Physiology , CNR , Pisa , Italy.
Romina D'Auriziod IIT , CNR , Pisa , Italy.ORCID 0000-0002-1728-6397
Sebastian Venckene Department of Clinical Microbiology , Royal College of Surgeon in Ireland , Dublin , Ireland.ORCID 0000-0003-1427-5657
Serena Meroa Institute of Clinical Physiology , CNR , Pisa , Italy.ORCID 0000-0003-2735-6990
Elena Guzzolinoa Institute of Clinical Physiology , CNR , Pisa , Italy.
Milena Rizzoa Institute of Clinical Physiology , CNR , Pisa , Italy.ORCID 0000-0001-5924-8615
Letizia Pittoa Institute of Clinical Physiology , CNR , Pisa , Italy.ORCID 0000-0002-8660-9516
Marco Pellegrinid IIT , CNR , Pisa , Italy.ORCID 0000-0003-3151-9481
Giovanna Chiorinof Cancer Genomics Lab , Fondazione Edo ed Elvo Tempia , Biella , Italy.ORCID 0000-0002-9502-6400
Catherine M Greenee Department of Clinical Microbiology , Royal College of Surgeon in Ireland , Dublin , Ireland.ORCID 0000-0003-2549-2569
Laura Polisenoa Institute of Clinical Physiology , CNR , Pisa , Italy.ORCID 0000-0001-6557-955X
Istituto di Fisiologia Clinica · ITInstitute of Informatics and Telematics · ITRoyal College of Surgeons in Ireland · IEFondazione Edo ed Elvo Tempia · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here we present miR-CATCHv2.0, an implemented experimental method that allows the identification of the microRNA species directly bound to an RNA of interest. After cross-linking of microRNA::RNA::Ago2 complexes using formaldehyde, the RNA is fragmented using sonication and then subjected to affinity purification using two sets of biotinylated tiling probes (ODD and EVEN). Finally, enriched microRNA species are retrieved by means of small RNA sequencing coupled with an

Indexed as

SoftwareHumansMicroRNAsProtein IsoformsProto-Oncogene Proteins B-rafReproducibility of ResultsRNA, MessengerRNA StabilityMicroRNAsProtein IsoformsProto-Oncogene Proteins B-rafRNA, Messengeraffinity purificationDirect microRNA-target bindingERK signallingmelanomatranscript stabilitytranscript translation-X1 mRNA

Identifiers

PMID30929607
PMCPMC6546394
OpenAlexW2933362900

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.