ArticleMolecular neurobiology2019
Prominent Postsynaptic and Dendritic Exocytosis of Endogenous BDNF Vesicles in BDNF-GFP Knock-in Mice.
Article in Molecular neurobiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Retrograde adenosine/ACell reports · 2024Article
- CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue.BMC biology · 2023Article
- Article
- The Role of BDNF as a Biomarker in Cognitive and Sensory Neurodegeneration.Journal of personalized medicine · 2023Review
- Neurotrophin signalling in the human nervous system.Frontiers in molecular neuroscience · 2023Review
- Stress-Related Dysfunction of Adult Hippocampal Neurogenesis-An Attempt for Understanding Resilience?International journal of molecular sciences · 2021Review
- Function of Drosophila Synaptotagmins in membrane trafficking at synapses.Cellular and molecular life sciences : CMLS · 2021Review
- Long-term depression at hippocampal mossy fiber-CA3 synapses involves BDNF but is not mediated by p75NTR signaling.Scientific reports · 2021Article
- The physiology of regulated BDNF release.Cell and tissue research · 2020Review
- Lactate and BDNF: Key Mediators of Exercise Induced Neuroplasticity?Journal of clinical medicine · 2020Review
- BDNF impact on synaptic dynamics: extra or intracellular long-term release differently regulates cultured hippocampal synapses.Molecular brain · 2020Article
- Genetic Increases in Olfactory Bulb BDNF Do Not Enhance Survival of Adult-Born Granule Cells.Chemical senses · 2020Article
- Article
- Direct current stimulation boosts hebbian plasticity in vitro.Brain stimulationArticle
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
Abstract
Brain-derived neurotrophic factor (BDNF) is a secreted messenger molecule that is crucial for neuronal function and induction of synaptic plasticity. Although altered availability of BDNF underlies many neurological deficits and neurodegenerative disorders, secretion dynamics of endogenous BDNF are unexplored. We generated a BDNF-GFP knock-in (KiBE) mouse, in which GFP-labeled BDNF is expressed under the control of the unaltered endogenous mouse BDNF gene regulatory elements. This KiBE mouse model enables for the first time live cell imaging analysis of endogenous BDNF dynamics. We show that BDNF-GFP release and biological activity in vivo are unaffected by the GFP tag, since homozygous KiBE mice, which lack wild-type BDNF, are healthy and have a normal life expectancy. STED superresolution microscopy shows that 70% of BDNF-GFP vesicles in KiBE mouse neurites are localized in dendrites, being typically 200 nm away from synaptic release sites. Live cell imaging in hippocampal slices also reveals prominent targeting of endogenous BDNF-GFP vesicles to dendrites. Fusion pore opening and cargo release of dendritic BDNF vesicles start within 30 s after a strong depolarizing stimulus and continue for > 100 s thereafter, revealing an astonishingly delayed and prolonged release of endogenous BDNF.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.