ArticleBiology of reproduction2019
Formation of organotypic testicular organoids in microwell culture†.
Article in Biology of reproduction, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed.
- Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026Review
- Associations of Bovine Fetal Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Germ Cell Marker Expression in Testicular Organoids.Animals : an open access journal from MDPI · 2026Article
- Induction of male neogametogenesis in a three-dimensional microenvironment supporting successful fertilization and proper embryo development.Molecular human reproduction · 2026Article
- Review
- Tuning the Testicular Microenvironment for Enhancing Human Sertoli Cells Maturation and Functionality In Vitro.Advanced healthcare materials · 2026Article
- Ferroptosis and Wnt/β-Catenin Signaling Triggered by Environmentally Relevant Nanoscale Polypropylene Plastics in Human Intestinal Models.ACS environmental Au · 2026Article
- Testicular organoid models in male reproductive toxicology: advances, applications, and future perspectives.Frontiers in physiology · 2026Article
- Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- [Research progress on the roles of primary cilia in fertility].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2025Review
- Construction of testicular organoids and their applications in the field of toxicology.Archives of toxicology · 2025Review
- In vitro models of the male reproductive system: applications for developmental and reproductive toxicology.Toxicological sciences : an official journal of the Society of Toxicology · 2025Review
- Melatonin Rescues Heat Stress-Induced Suppression of TCA Cycle and Mitochondrial Damage in Goat Sertoli Cells.International journal of molecular sciences · 2025Article
- Platelet-derived growth factor signaling in porcine testicular tubular somatic cells.Cell and tissue research · 2025Article
- Male fertility restoration with stem cell-based therapies: from testicular stem cells to pluripotent stem cell-based in vitro gametogenesis.Fertility and sterility · 2025Review
- Artificial Gametogenesis and In Vitro Spermatogenesis: Emerging Strategies for the Treatment of Male Infertility.International journal of molecular sciences · 2025Review
- Identification and isolation of human testicular peritubular myoid cells and Leydig cells by a combination of ITGA9 and NGFR.Reproductive biology and endocrinology : RB&E · 2025Article
- New insights into the testicular tropism of porcine reproductive and respiratory syndrome virus.Microbiology spectrum · 2025Article
- A new humanHuman reproduction open · 2025Article
- Individualization of Testicular Cells for Single-Cell Subcellular Immunocytochemistry and Cell Culture Applications.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Generation of Organotypic Testicular Organoids from Rat and Pig Primary Cells in Microwell Culture.Methods in molecular biology (Clifton, N.J.) · 2025Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
Abstract
Three-dimensional (3D) organoids can serve as an in vitro platform to study cell-cell interactions, tissue development, and toxicology. Development of organoids with tissue architecture similar to testis in vivo has remained a challenge. Here, we present a microwell aggregation approach to establish multicellular 3D testicular organoids from pig, mouse, macaque, and human. The organoids consist of germ cells, Sertoli cells, Leydig cells, and peritubular myoid cells forming a distinct seminiferous epithelium and interstitial compartment separated by a basement membrane. Sertoli cells in the organoids express tight junction proteins claudin 11 and occludin. Germ cells in organoids showed an attenuated response to retinoic acid compared to germ cells in 2D culture indicating that the tissue architecture of the organoid modulates response to retinoic acid similar to in vivo. Germ cells maintaining physiological cell-cell interactions in organoids also had lower levels of autophagy indicating lower levels of cellular stress. When organoids were treated with mono(2-ethylhexyl) phthalate (MEHP), levels of germ cell autophagy increased in a dose-dependent manner, indicating the utility of the organoids for toxicity screening. Ablation of primary cilia on testicular somatic cells inhibited the formation of organoids demonstrating an application to screen for factors affecting testicular morphogenesis. Organoids can be generated from cryopreserved testis cells and preserved by vitrification. Taken together, the testicular organoid system recapitulates the 3D organization of the mammalian testis and provides an in vitro platform for studying germ cell function, testicular development, and drug toxicity in a cellular context representative of the testis in vivo.
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