ArticleMolecular neurobiology2019
Reference Gene Validation via RT-qPCR for Human iPSC-Derived Neural Stem Cells and Neural Progenitors.
Article in Molecular neurobiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
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- Digital PCR-Based Gene Expression Analysis Using a Highly Multiplexed Assay with Universal Detection Probes to Study Induced Pluripotent Stem Cell Differentiation into Cranial Neural Crest Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Quality assessment strategy development and analytical method selection of GMP grade biological drugs for gene and cell therapy.BBA advances · 2025Review
- Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons.iScience · 2023Article
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- Immune-related lncRNA pairs as novel signature to predict prognosis and immune landscape in melanoma patients.Medicine · 2022Article
- Synthesis of Dolichols inInternational journal of molecular sciences · 2021Article
- Rational, Unbiased Selection of Reference Genes for Pluripotent Stem Cell-Derived Cardiomyocytes.Tissue engineering. Part C, Methods · 2021Article
- Resident bacteria contribute to opportunistic infections of the respiratory tract.PLoS pathogens · 2021Article
- Detection of Pancreatic Ductal Adenocarcinoma by A qPCR-based Normalizer-free Circulating Extracellular Vesicles RNA Signature.Journal of Cancer · 2021Article
- Zinc and Copper Brain Levels and Expression of Neurotransmitter Receptors in Two Rat ASD Models.Frontiers in molecular neuroscience · 2021Article
- Changes in the Expression of SNAP-25 Protein in the Brain of Juvenile Rats in Two Models of Autism.Journal of molecular neuroscience : MN · 2020Article
- Reference Gene Validation in the Brain Regions of Young Rats after Pentylenetetrazole-Induced Seizures.Biomedicines · 2020Article
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5 authors.
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Abstract
Correct selection of the reference gene(s) is the most important step in gene expression analysis. The aims of this study were to identify and evaluate the panel of possible reference genes in neural stem cells (NSC), early neural progenitors (eNP) and neural progenitors (NP) obtained from human-induced pluripotent stem cells (hiPSC). The stability of expression of genes commonly used as the reference in cells during neural differentiation is variable and does not meet the criteria for reference genes. In the present work, we evaluated the stability of expression of 16 candidate reference genes using the four most popular algorithms: the ΔCt method, BestKeeper, geNorm and NormFinder. All data were analysed using the online tool RefFinder to obtain a comprehensive ranking. Our results indicate that NormFinder is the best tool for reference gene selection in early stages of hiPSC neural differentiation. None of the 16 tested genes is suitable as reference gene for all three stages of development. We recommend using different genes (panel of genes) to normalise RT-qPCR data for each of the neural differentiation stages.
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