ArticleNucleic acids research2019
PIXUL-ChIP: integrated high-throughput sample preparation and analytical platform for epigenetic studies.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
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- Automated chromatin profiling with spa-ChIP-seq uncovers the impacts of condition variations.bioRxiv : the preprint server for biology · 2025Article
- Identification of functional non-coding variants associated with orofacial cleft.Nature communications · 2025Article
- An innovative high-throughput genome releaser for rapid and efficient PCR screening.Frontiers in bioengineering and biotechnology · 2025Article
- Analysis of DNA Methylation in Gliomas: Assessment of Preanalytical Variables.Laboratory investigation; a journal of technical methods and pathology · 2024Article
- High incidence and geographic distribution of cleft palate in Finland are associated with the IRF6 gene.Nature communications · 2024Article
- Regulation of versican expression in macrophages is mediated by canonical type I interferon signaling via ISGF3.American journal of physiology. Cell physiology · 2024Article
- Regulation of Versican Expression in Macrophages is Mediated by Canonical Type I Interferon Signaling via ISGF3.bioRxiv : the preprint server for biology · 2024Article
- [Development of a high intensity focused ultrasound device for bio-samples preparation].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2024Article
- A High-Throughput PIXUL-Matrix-Based Toolbox to Profile Frozen and Formalin-Fixed Paraffin-Embedded Tissues Multiomes.Laboratory investigation; a journal of technical methods and pathology · 2024Article
- CryoGrid-PIXUL-RNA: high throughput RNA isolation platform for tissue transcript analysis.BMC genomics · 2023Article
- ASAP─Automated Sonication-Free Acid-Assisted Proteomes─from Cells and FFPE Tissues.Analytical chemistry · 2023Article
- DNA fragmentation in a steady shear flow.Biomicrofluidics · 2022Article
- dCas9 fusion to computer-designed PRC2 inhibitor reveals functional TATA box in distal promoter region.Cell reports · 2022Article
- Increased apolipoprotein C3 drives cardiovascular risk in type 1 diabetes.The Journal of clinical investigation · 2019Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Chromatin immunoprecipitation (ChIP) is the most widely used approach for identification of genome-associated proteins and their modifications. We have previously introduced a microplate-based ChIP platform, Matrix ChIP, where the entire ChIP procedure is done on the same plate without sample transfers. Compared to conventional ChIP protocols, the Matrix ChIP assay is faster and has increased throughput. However, even with microplate ChIP assays, sample preparation and chromatin fragmentation (which is required to map genomic locations) remains a major bottleneck. We have developed a novel technology (termed 'PIXUL') utilizing an array of ultrasound transducers for simultaneous shearing of samples in standard 96-well microplates. We integrated PIXUL with Matrix ChIP ('PIXUL-ChIP'), that allows for fast, reproducible, low-cost and high-throughput sample preparation and ChIP analysis of 96 samples (cell culture or tissues) in one day. Further, we demonstrated that chromatin prepared using PIXUL can be used in an existing ChIP-seq workflow. Thus, the high-throughput capacity of PIXUL-ChIP provides the means to carry out ChIP-qPCR or ChIP-seq experiments involving dozens of samples. Given the complexity of epigenetic processes, the use of PIXUL-ChIP will advance our understanding of these processes in health and disease, as well as facilitate screening of epigenetic drugs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.