ArticleNucleic acids research2019
In vitro isolation of class-specific oligonucleotide-based small-molecule receptors.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 76 citations in OpenAlex.
- A Selective, Class-Specific Aptamer for Fentanyl Analog Screening.Analytical chemistry · 2026Article
- Article
- Article
- Reagentless Real-Time ATP Monitoring with New DNA Aptamers.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Approaches for Identifying LncRNA-Associated Proteins for Therapeutic Targets and Cancer Biomarker Discovery.Targets (Basel) · 2025Article
- Progress in Aptamer Research and Future Applications.ChemistryOpen · 2025Review
- Exploring the relationship between aptamer binding thermodynamics, affinity, and specificity.Nucleic acids research · 2025Article
- Improving Aptamer Affinity and Determining Sequence-Activity Relationships via Motif-SELEX.Journal of the American Chemical Society · 2025Article
- A Label-Free Colorimetric Aptasensor for Flavokavain B Detection.Sensors (Basel, Switzerland) · 2025Article
- Examining the Relationship between Aptamer Complexity and Molecular Discrimination of a Low-Epitope Target.ACS central science · 2024Article
- Developing Aptamer-Based Colorimetric Opioid Tests.JACS Au · 2024Article
- High-Affinity Aptamers forJournal of the American Chemical Society · 2024Article
- Screening biotoxin aptamer and their application of optical aptasensor in food stuff: a review.Frontiers in chemistry · 2024Review
- A functional group-guided approach to aptamers for small molecules.Science (New York, N.Y.) · 2023Article
- High-throughput quantitative binding analysis of DNA aptamers using exonucleases.Nucleic acids research · 2023Article
- Critical Design Factors for Electrochemical Aptasensors Based on Target-Induced Conformational Changes: The Case of Small-Molecule Targets.Biosensors · 2022Review
- A system for multiplexed selection of aptamers with exquisite specificity without counterselection.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Recent Advances in Micro/Nanomaterial-Based Aptamer Selection Strategies.Molecules (Basel, Switzerland) · 2021Review
- Advances and Challenges in Small-Molecule DNA Aptamer Isolation, Characterization, and Sensor Development.Angewandte Chemie (International ed. in English) · 2021Review
- Immobilization Strategies for Enhancing Sensitivity of Electrochemical Aptamer-Based Sensors.ACS applied materials & interfaces · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Class-specific bioreceptors are highly desirable for recognizing structurally similar small molecules, but the generation of such affinity elements has proven challenging. We here develop a novel 'parallel-and-serial' selection strategy for isolating class-specific oligonucleotide-based receptors (aptamers) in vitro. This strategy first entails parallel selection to selectively enrich cross-reactive binding sequences, followed by serial selection that enriches aptamers binding to a designated target family. As a demonstration, we isolate a class-specific DNA aptamer against a family of designer drugs known as synthetic cathinones. The aptamer binds to 12 diverse synthetic cathinones with nanomolar affinity and does not respond to 11 structurally similar non-target compounds, some of which differ from the cathinone targets by a single atom. This is the first account of an aptamer exhibiting a combination of broad target cross-reactivity, high affinity and remarkable specificity. Leveraging the qualities of this aptamer, instantaneous colorimetric detection of synthetic cathinones at nanomolar concentrations in biological samples is achieved. Our findings significantly expand the binding capabilities of aptamers as class-specific bioreceptors and further demonstrate the power of rationally designed selection strategies for isolating customized aptamers with desired binding profiles. We believe that our aptamer isolation approach can be broadly applied to isolate class-specific aptamers for various small molecule families.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.