ArticleScientific reports2019
Novel combination of feed enzymes to improve the degradation of Chlorella vulgaris recalcitrant cell wall.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 74 citations in OpenAlex.
- Enzymatic Hydrolysates fromMarine drugs · 2026Article
- Recent Advances in NADES-Assisted Process Intensification Technologies for Sustainable Recovery of Microalgal Bioactives: Challenges and Future Prospectives.Marine drugs · 2026Review
- Chlorella vulgaris mutants with altered cell walls show increased permeability and enhanced extractability of intracellular molecules.Biotechnology for biofuels and bioproducts · 2025Article
- Protein Extraction fromJournal of microbiology and biotechnology · 2025Article
- Functional and Bioactive Benefits of Selected Microalgal Hydrolysates Assessed In Silico and In Vitro.Marine drugs · 2025Article
- Improving protein hydrolysis and digestibility inHeliyon · 2025Article
- Metabolomics and proteomics insights into hepatic responses of weaned piglets to dietary Spirulina inclusion and lysozyme supplementation.BMC veterinary research · 2024Article
- Assessing the Influence of CumulativeFoods (Basel, Switzerland) · 2024Review
- Improving protein extraction and peptide production fromHeliyon · 2024Article
- Microalgae as an Alternative Mineral Source in Poultry Nutrition.Veterinary sciences · 2024Review
- Effects of partial replacement of soybean meal withFrontiers in veterinary science · 2024Article
- Microalgae as a Sustainable Source of Antioxidants in Animal Nutrition, Health and Livestock Development.Antioxidants (Basel, Switzerland) · 2023Review
- Enhancing Digestibility ofAnimals : an open access journal from MDPI · 2023Review
- Integrated Omics analysis of pig muscle metabolism under the effects of dietary Chlorella vulgaris and exogenous enzymes.Scientific reports · 2022Article
- Impact of dietary Chlorella vulgaris and feed enzymes on health status, immune response and liver metabolites in weaned piglets.Scientific reports · 2022Article
- Effect on Broiler Production Performance and Meat Quality of FeedingAnimals : an open access journal from MDPI · 2022Article
- Recalcitrant cell wall ofAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Influence of Chlorella vulgaris on growth, digestibility and gut morphology and microbiota of weaned piglet.Scientific reports · 2022Article
- Testimony on a successful lab protocol to disrupt Chlorella vulgaris microalga cell wall.PloS one · 2022Article
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, a rational combination of 200 pre-selected Carbohydrate-Active enzymes (CAZymes) and sulfatases were tested, individually or combined, according to their ability to degrade Chlorella vulgaris cell wall to access its valuable nutritional compounds. The disruption of microalgae cell walls by a four-enzyme mixture (Mix) in comparison with the control, enabled to release up to 1.21 g/L of reducing sugars (p < 0.001), led to an eight-fold increase in oligosaccharides release (p < 0.001), and reduced the fluorescence intensity by 47% after staining with Calcofluor White (p < 0.001). The Mix treatment was successful in releasing proteins (p < 0.001), some MUFA (p < 0.05), and the beneficial 18:3n-3 fatty acid (p < 0.05). Even if no variation was detected for chlorophylls (p > 0.05), total carotenoids were increased in the supernatant (p < 0.05) from the Mix treatment, relative to the control. Taken together, these results indicate that this four-enzyme Mix displays an effective capacity to degrade C. vulgaris cell wall. Thus, these enzymes may constitute a good approach to improve the bioavailability of C. vulgaris nutrients for monogastric diets, in particular, and to facilitate the cost-effective use of microalgae by the feed industry, in general.
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