ArticleApplied and environmental microbiology2019
Development of a CRISPR/Cas9 System for Methylococcus capsulatus
Article in Applied and environmental microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 57 citations in OpenAlex.
- Evading native restriction-modification systems improves electroporation efficiency of the methanotrophApplied and environmental microbiology · 2026Article
- Advances in biotechnological methods for genetic and metabolic engineering in Methylomonas sp. DH-1.Journal of biological engineering · 2026Review
- CRISPRi-based functional genomic screening identifies genes essential for CHScience advances · 2026Article
- Biological conversion of methane to organic molecules: towards a low-carbon bioeconomy.National science review · 2026Review
- Methanol biotransformation for the production of biodegradable plastic monomer L-lactate in yeast.Nature communications · 2025Article
- Advancements in the production of value-added products via methane biotransformation by methanotrophs: Current status and future perspectives.Journal of microbiology (Seoul, Korea) · 2025Review
- Overexpression of native carbonic anhydrases increases carbon conversion efficiency in the methanotrophic biocatalystmSphere · 2024Article
- The elimination of two restriction enzyme genes allows for electroporation-based transformation and CRISPR-Cas9-based base editing in the non-competent Gram-negative bacteriumApplied and environmental microbiology · 2024Article
- Construction of a broad-host-range Anderson promoter series and particulate methane monooxygenase promoter variants expand the methanotroph genetic toolbox.Synthetic and systems biotechnology · 2024Article
- Direct Methane Oxidation by Copper- and Iron-Dependent Methane Monooxygenases.Chemical reviews · 2024Review
- Systematic Part Transfer by Extending a Modular Toolkit to Diverse Bacteria.ACS synthetic biology · 2023Article
- In Vivo Genome Editing in Type I and II Methanotrophs Using a CRISPR/Cas9 System.ACS synthetic biology · 2023Article
- Cell-Free Protein Synthesis of Particulate Methane Monooxygenase into Nanodiscs.ACS synthetic biology · 2022Article
- Transcription regulation strategies in methylotrophs: progress and challenges.Bioresources and bioprocessing · 2022Review
- Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant BacteriumACS synthetic biology · 2022Article
- Optimized Tools and Methods for Methanotroph Genome Editing.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RubisCO) Is Essential for Growth of the Methanotroph Methylococcus capsulatus Strain Bath.Applied and environmental microbiology · 2021Article
- Genome Editing in Bacteria: CRISPR-Cas and Beyond.Microorganisms · 2021Review
- Biochemistry of aerobic biological methane oxidation.Chemical Society reviews · 2021Review
- Two CRISPR/Cas9 Systems Developed in Thermomyces dupontii and Characterization of Key Gene Functions in Thermolide Biosynthesis and Fungal Adaptation.Applied and environmental microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methanotrophic bacteria play a crucial role in the Earth's biogeochemical cycle and have the potential to be employed in industrial biomanufacturing processes due to their capacity to use natural gas- and biogas-derived methane as a sole carbon and energy source. Advanced gene-editing systems have the potential to enable rapid, high-throughput methanotrophic genetics and biocatalyst development. To this end, we employed a series of broad-host-range expression plasmids to construct a conjugatable
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.