ArticleMolecules (Basel, Switzerland)2019
Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology.
Article in Molecules (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Marine-derived PUFAs and peptides for aging-related memory deficits: processing methods, pharmacokinetics, mechanistic insights, and clinical application.Frontiers in nutrition · 2026Review
- Non-steroidal anti-inflammatory drugs conjugated to a synthetic peptide exhibitRSC medicinal chemistry · 2025Article
- Exploring New Biomarkers for Detecting Whey Adulteration in Milk: Synthesis and Characterization of Caseinomacropeptide-Derived Peptides.ACS omega · 2025Article
- Self-Assembly of a Novel Pentapeptide into Hydrogelated Dendritic Architecture: Synthesis, Properties, Molecular Docking and Prospective Applications.Gels (Basel, Switzerland) · 2024Article
- Evaluating theACS omega · 2023Article
- Non-natural amino acids into LfcinB-derived peptides: effect in their (i) proteolytic degradation and (ii) cytotoxic activity against cancer cells.Royal Society open science · 2023Article
- Changes in Length and Positive Charge of Palindromic Sequence RWQWRWQWR Enhance Cytotoxic Activity against Breast Cancer Cell Lines.ACS omega · 2023Article
- Efficient Separation of C-Tetramethylcalix[4]resorcinarene Conformers by Means of Reversed-Phase Solid-Phase Extraction.ACS omega · 2023Article
- Gradient Retention Factor Concept Applied to Method Development for Peptide Analysis by Means of RP-HPLC.ACS omega · 2022Article
- Review
- Evidence of Isomerization in the Michael-Type Thiol-Maleimide Addition: Click Reaction between L-Cysteine and 6-Maleimidehexanoic Acid.Molecules (Basel, Switzerland) · 2022Article
- An Updated Review of Bioactive Peptides from Mushrooms in a Well-Defined Molecular Weight Range.Toxins · 2022Review
- Obtaining an immunoaffinity monolithic material: poly(GMA-RSC advances · 2021Article
- Article
- Peptides Derived from (RRWQWRMKKLG)International journal of molecular sciences · 2020Article
- Shorter Antibacterial Peptide Having High Selectivity forMicroorganisms · 2020Article
- Selective cytotoxic effect against the MDA-MB-468 breast cancer cell line of the antibacterial palindromic peptide derived from bovine lactoferricin.RSC advances · 2020Article
- The tetrameric peptide LfcinB (20-25)RSC advances · 2019Article
- Pullulan nanofibers containing the antimicrobial palindromic peptide LfcinB (21-25)RSC advances · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A methodology was implemented for purifying peptides in one chromatographic run via solid-phase extraction (SPE), reverse phase mode (RP), and gradient elution, obtaining high-purity products with good yields. Crude peptides were analyzed by reverse phase high performance liquid chromatography and a new mathematical model based on its retention time was developed in order to predict the percentage of organic modifier in which the peptide will elute in RP-SPE. This information was used for designing the elution program of each molecule. It was possible to purify peptides with different physicochemical properties, showing that this method is versatile and requires low solvent consumption, making it the least polluting one. Reverse phase-SPE can easily be routinely implemented. It is an alternative to enrich and purified synthetic or natural molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.