ArticleEndocrine connections2019
Elevated free fatty acids affect bovine granulosa cell function: a molecular cue for compromised reproduction during negative energy balance.
Article in Endocrine connections, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Lipid dysregulation in mouse ovarian antral follicles after oral exposure to a human relevant mixture of three phthalates.Toxicology · 2026Article
- Dietary Supplementation with Raspberry or Strawberry Seed Oil Impacts Folliculogenesis, Hormonal Parameters and the Fatty Acid Profile in the Juvenile Rabbit Ovary.Animals : an open access journal from MDPI · 2026Article
- PDK4 is critical for metabolic regulation and progesterone production in bovine granulosa cells†.Biology of reproduction · 2026Article
- Metabolic and endocrinologic interplay in the peri-ovulatory follicle to support the cumulus-oocyte-complex towards full competence.Animal reproduction · 2025Review
- Obesity-Related Hypogonadism in Women.Endocrine reviews · 2024Review
- Role of functional fatty acids in modulation of reproductive potential in livestock.Journal of animal science and biotechnology · 2023Review
- High-Fat Diet and Female Fertility across Lifespan: A Comparative Lesson from Mammal Models.Nutrients · 2022Review
- Seasonal Variations in the Lipid Profile of the Ovarian Follicle in Italian Mediterranean Buffaloes.Animals : an open access journal from MDPI · 2022Article
- Chitosan/Calcium-Alginate Encapsulated Flaxseed Oil on Dairy Cattle Diet: In Vitro Fermentation and Fatty Acid Biohydrogenation.Animals : an open access journal from MDPI · 2022Article
- Metabolism of fatty acids in follicular cells, oocytes, and blastocysts.Reproduction & fertility · 2022Review
- Oleic acid reduces steroidogenesis by changing the lipid type stored in lipid droplets of ovarian granulosa cells.Journal of animal science and biotechnology · 2022Article
- Impact of fibronectin type III domain-containing family in the changes in metabolic and hormonal profiles during peripartum period in dairy cows.Frontiers in veterinary science · 2022Article
- Gene cascade analysis in human granulosa tumor cells (KGN) following exposure to high levels of free fatty acids and insulin.Journal of ovarian research · 2021Article
- Article
- Cocultured porcine granulosa cells respond to excess non-esterified fatty acids during in vitro maturation.Journal of ovarian research · 2021Article
- Genome wide effects of oleic acid on cultured bovine granulosa cells: evidence for the activation of pathways favoring folliculo-luteal transition.BMC genomics · 2021Article
- Non-esterified fatty acids in the ovary: friends or foes?Reproductive biology and endocrinology : RB&E · 2020Review
- Effects of Dietary Fatty Acids on Bovine Oocyte Competence and Granulosa Cells.Frontiers in endocrinology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-yielding dairy cows postpartum face the challenge of negative energy balance leading to elevated free fatty acids levels in the serum and follicular fluid thus affecting the ovarian function. Here, we investigated effects of physiological concentrations of palmitic acid (PA), stearic acid (SA) and oleic acid (OA) on the viability, steroid production and gene expression in a bovine granulosa cell (GC) culture model. Treatment with individual and combined fatty acids increased the CD36 gene expression, while no significant apoptotic effects were observed. Both PA and SA significantly upregulated the expression of FSHR, LHCGR, CYP19A1, HSD3B1, CCND2 and increased 17β-estradiol (E2) production, while OA downregulated the expression of these genes and reduced E2. Interestingly, STAR was equally downregulated by all fatty acids and combination treatment. E2 was significantly reduced after combination treatment. To validate the effects of OA, in vivo growing dominant follicles (10-19 mm) were injected with bovine serum albumin (BSA) with/without conjugated OA. The follicular fluid was recovered 48 h post injection. As in our in vitro model, OA significantly reduced intrafollicular E2 concentrations. In addition, expression of CD36 was significantly up- and that of CYP19A1 and STAR significantly downregulated in antral GC recovered from aspirated follicles. The ovulation rates of OA-injected follicles tended to be reduced. Our results indicate that elevated free fatty acid concentrations specifically target functional key genes in GC both in vitro and in vivo. Suggestively, this could be a possible mechanism through which elevated free fatty acids affect folliculogenesis in dairy cows postpartum.
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