ArticlePLoS pathogens2019
RNAi-mediated knockdown of daf-12 in the model parasitic nematode Strongyloides ratti.
Article in PLoS pathogens, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
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- Using newly optimized genetic tools to probe Strongyloides sensory behaviors.Molecular and biochemical parasitology · 2022Review
- Opinion: What do rescue experiments with heterologous proteins tell us and what not?Parasitology research · 2022Article
- On the role of dauer in the adaptation of nematodes to a parasitic lifestyle.Parasites & vectors · 2021Review
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- Identification of a nuclear receptor/coactivator developmental signaling pathway in the nematode parasiteProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Rhabditophanes diutinus a parthenogenetic clade IV nematode with dauer larvae.PLoS pathogens · 2020Article
- Chitin Biosynthesis Inhibition ofInternational journal of molecular sciences · 2020Article
- Correction: RNAi-mediated knockdown of daf-12 in the model parasitic nematode Strongyloides ratti.PLoS pathogens · 2020Article
- Review
- The role of carbon dioxide in nematode behaviour and physiology.Parasitology · 2020Review
- Recent advances in functional genomics for parasitic nematodes of mammals.The Journal of experimental biology · 2020Review
- Worming our way toward multiple evolutionary origins of convergent sterol pathways.Journal of lipid research · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene daf-12 has long shown to be involved in the dauer pathway in Caenorhabditis elegans (C. elegans). Due to the similarities of the dauer larvae of C. elegans and infective larvae of certain parasitic nematodes such as Strongyloides spp., this gene has also been suspected to be involved in the development of infective larvae. Previous research has shown that the application of dafachronic acid, the steroid hormone ligand of DAF-12 in C. elegans, affects the development of infective larvae and metabolism in Strongyloides. However, a lack of tools for either forward or reverse genetics within Strongyloides has limited studies of gene function within these important parasites. After determining whether Strongyloides had the requisite proteins for RNAi, we developed and report here the first successful RNAi by soaking protocol for Strongyloides ratti (S. ratti) and use this protocol to study the functions of daf-12 within S. ratti. Suppression of daf-12 in S. ratti severely impairs the formation of infective larvae of the direct cycle and redirects development towards the non-infective (non-dauer) free-living life cycle. Further, daf-12(RNAi) S. ratti produce slightly but significantly fewer offspring and these offspring are developmentally delayed or incapable of completing their development to infective larvae (L3i). Whilst the successful daf-12(RNAi) L3i are still able to infect a new host, the resulting infection is less productive and shorter lived. Further, daf-12 knockdown affects metabolism in S. ratti resulting in a shift from aerobic towards anaerobic fat metabolism. Finally, daf-12(RNAi) S. ratti have reduced tolerance of temperature stress.
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