ArticleBritish journal of pharmacology2019
Influence of sildenafil on the purinergic components of nerve-mediated and urothelial ATP release from the bladder of normal and spinal cord injured mice.
Article in British journal of pharmacology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Pathophysiology of sildenafil-induced ocular toxicity in rats and treatment.International journal of ophthalmology · 2026Article
- Investigation of image-guided in vivo irradiation on voiding patterns and bladder contractility in female mice.Scientific reports · 2025Article
- Can the Bladder Itself "Measure" Volume, and Thereby Help to Determine When Initiation of Voiding Should Occur? ICI-RS 2024.Neurourology and urodynamics · 2025Review
- New therapeutic targets to prevent benign prostatic enlargement and symptomatic progression to benign prostatic obstruction-ICI-RS 2023.Neurourology and urodynamics · 2024Review
- PDE5 inhibitor potentially improves polyuria and bladder storage and voiding dysfunctions in type 2 diabetic rats.PloS one · 2024Article
- Excitatory purinergic and cholinergic expression changed in a partial bladder outlet obstruction-induced overactive bladder rat model.Scientific reports · 2023Article
- Soluble Guanylate Cyclase Activators to Treat Benign Prostatic Hyperplasia and associated LUTS.Continence (Amsterdam, Netherlands) · 2023Article
- Targeting neurotrophin and nitric oxide signaling to promote recovery and ameliorate neurogenic bladder dysfunction following spinal cord injury - Mechanistic concepts and clinical implications.Continence (Amsterdam, Netherlands) · 2023Article
- Soluble guanylate cyclase mediates the relaxation of healthy and inflamed bladder smooth muscle by aqueous nitric oxide.Frontiers in physiology · 2023Article
- Selective reduction of neurotransmitter release by cAMP-dependent pathways in mouse detrusor.American journal of physiology. Regulatory, integrative and comparative physiology · 2022Article
- Frequency-dependent characteristics of nerve-mediated ATP and acetylcholine release from detrusor smooth muscle.Experimental physiology · 2022Article
- Drugs Currently Undergoing Preclinical or Clinical Trials for the Treatment of Overactive Bladder: A Review.Current therapeutic research, clinical and experimental · 2022Review
- Urothelial Calcium-Sensing Receptor Modulates Micturition Function via Mediating Detrusor Activity and Ameliorates Bladder Hyperactivity in Rats.Pharmaceuticals (Basel, Switzerland) · 2021Article
- The Frequency-Dependence of Pre- and Postganglionic Nerve Stimulation of Pig and Rat Bladder.International neurourology journal · 2021Article
- The nitric oxide-cyclic guanosine monophosphate pathway inhibits the bladder ATP release in response to a physiological or pathological stimulus.Physiological reports · 2021Article
- New targets for overactive bladder-ICI-RS 2109.Neurourology and urodynamics · 2020Review
- Characterisation of nerve-mediated ATP release from bladder detrusor muscle and its pathological implications.British journal of pharmacology · 2019Article
- Influence of sildenafil on the purinergic components of nerve-mediated and urothelial ATP release from the bladder of normal and spinal cord injured mice.British journal of pharmacology · 2019Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
background and purposePDE inhibitors such as sildenafil alleviate lower urinary tract symptoms; however, a complete understanding of their action on the bladder remains unclear. We are investigating the effects of sildenafil, on post and preganglionic nerve-mediated contractions of the mouse bladder, and neuronal and urothelial ATP release. EXPERIMENTAL APPROACH: Bladders were used from young (12 weeks), aged (24 months), and spinal cord transected (SCT), mice, for in vitro contractility experiments. An arterially perfused in situ whole mouse model was used to record bladder pressure. Nerve-mediated contractions were generated by electrical field stimulation (EFS) of postganglionic nerve terminals or the pelvic nerve. ATP release during EFS in intact detrusor strips, and during stretch of isolated mucosa strips, was measured using a luciferin-luciferase assay. KEY
resultsSildenafil (20 μM) inhibited nerve-mediated contractions in young mice, with an increase in f CONCLUSION AND IMPLICATIONS: Sildenafil has a greater effect on the low-frequency, purinergic-mediated contractions and suppresses neuronal ATP release. In addition, sildenafil reduces stretch-induced urothelial ATP release. These results demonstrate a novel action of sildenafil to selectively inhibit ATP release from nerve terminals innervating detrusor smooth muscle and the urothelium.
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