ArticleFungal biology and biotechnology2019
Development of microtiter plate scale CRISPR/Cas9 transformation method for
Article in Fungal biology and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- CRISPR-Cas9 Gene Editing inBiology · 2025Review
- Automated Genetic Manipulation for the Construction ofChem & bio engineering · 2025Article
- Optimization of the Genome Editing CRISPR-Cas9 Technology in Scedosporium apiospermum.Mycopathologia · 2025Article
- Recent Advances in Heterologous Protein Expression and Natural Product Synthesis byJournal of fungi (Basel, Switzerland) · 2025Review
- Metabolic engineering for microbial production of sugar acids.BMC biotechnology · 2025Review
- Considerations for Domestication of Novel Strains of Filamentous Fungi.ACS synthetic biology · 2025Review
- Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit.Nature communications · 2024Article
- Utilization of CRISPR-Cas genome editing technology in filamentous fungi: function and advancement potentiality.Frontiers in microbiology · 2024Review
- The Gal4-Type Transcription Factor Pro1 Integrates Inputs from Two Different MAPK Cascades to Regulate Development in the Fungal PathogenJournal of fungi (Basel, Switzerland) · 2022Article
- Manipulation of the Global RegulatorACS chemical biology · 2022Article
- Use of ambrAMB Express · 2022Article
- CRISPR/Cas9-Based Genome Editing and Its Application inJournal of fungi (Basel, Switzerland) · 2022Review
- Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi.Frontiers in bioengineering and biotechnology · 2022Review
- CRISPR/Cas9 RNP-assisted validation of palmarumycin biosynthetic gene cluster inFrontiers in microbiology · 2022Article
- Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum.PloS one · 2022Article
- CRISPR-based transcriptional activation tool for silent genes in filamentous fungi.Scientific reports · 2021Article
- Applications of CRISPR/Cas9 in the Synthesis of Secondary Metabolites in Filamentous Fungi.Frontiers in microbiology · 2021Review
- Metabolic engineering of Aspergillus niger via ribonucleoprotein-based CRISPR-Cas9 system for succinic acid production from renewable biomass.Biotechnology for biofuels · 2020Article
- A novel fungal gene regulation system based on inducible VPR-dCas9 and nucleosome map-guided sgRNA positioning.Applied microbiology and biotechnology · 2020Article
- Engineering marine fungi for conversion of D-galacturonic acid to mucic acid.Microbial cell factories · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe CRISPR/Cas9 is currently the predominant technology to enhance the genome editing efficiency in eukaryotes. Established tools for many fungal species exist while most of them are based on in vivo expressed Cas9 and guide RNA (gRNA). Alternatively, in vitro assembled Cas9 and gRNA ribonucleoprotein complexes can be used in genome editing, however, only a few examples have been reported in fungi. In general, high-throughput compatible transformation workflows for filamentous fungi are immature.
resultsIn this study, a CRISPR/Cas9 facilitated transformation and genome editing method based on in vitro assembled ribonucleoprotein complexes was developed for the filamentous fungus
conclusionsThe developed microtiter plate compatible CRISPR/Cas9 method provides a basis for high-throughput genome editing workflows in
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