ArticleBiotechnology for biofuels2019
New strategy to elucidate the positive effects of extractable lignin on enzymatic hydrolysis by quartz crystal microbalance with dissipation.
Article in Biotechnology for biofuels, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 61 citations in OpenAlex.
- Recent advances in understanding the effects of lignin structural characteristics on enzymatic hydrolysis.Biotechnology for biofuels · 2021Review
- Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility.Biotechnology for biofuels · 2021Article
- Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses.Bioresources and bioprocessing · 2021Article
- Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo.Frontiers in bioengineering and biotechnology · 2021Article
- Effect of Lignin Content on Cellulolytic Saccharification of Liquid Hot Water Pretreated Sugarcane Bagasse.Molecules (Basel, Switzerland) · 2020Article
- Boosting Ethanol Productivity ofFrontiers in microbiology · 2019Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
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Abstract
backgroundThe presence of lignin normally affects enzymatic saccharification of lignocellulose detrimentally. However, positive effects of lignin on enzymatic hydrolysis have been recently reported. Enzyme-lignin interactions could be the key to reveal the underlying mechanism of their discrepant behaviors. In this study, to elucidate the positive effects of extractable lignin (EL) on enzymatic hydrolysis of ethanol organosolv-pretreated wood sawdust, two lignin fractions, EL and milled wood lignin (MWL), were isolated sequentially from pretreated substrates. Quartz crystal microbalance with dissipation (QCM-D) was then used to investigate the lignin aggregation effects on enzyme adsorption.
resultsWe found that both EL and MWL had a narrow molecular weight distribution. However, MWL had an obviously higher molecular weight than EL. This indicated that EL and MWL likely represent two distinct lignin fractions from ethanol organosolv-pretreated substrates. HSQC NMR analysis revealed that less β-
conclusionsLignin structure determined the interaction between enzyme and lignins. A positive correlation was observed between molecular weight, the content of β-5 linkages, and enzyme adsorption on lignin. EL, which was more depolymerized and less condensed, had the lower enzyme adsorption of the two preparations tested. Additionally, the presence of EL reduced enzyme adsorption on reconstructed lignin films, perhaps through a mechanism involving the blocking of non-productive enzyme binding sites on the MWL. This could be the mechanism for the positive effects of EL on enzymatic hydrolysis.
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