ArticleNature methods2019
SNAC-tag for sequence-specific chemical protein cleavage.
Article in Nature methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
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- Small-molecule binding and sensing with a designed protein family.Nature communications · 2026Article
- SNAC-Tag Integration Into NPN Platform Facilitates Efficient Purification of Recombinant Proteins From Nicotiana benthamiana.Plant biotechnology journal · 2026Article
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- Boolean logic-gated protein presentation through autonomously compiled molecular topology.Nature chemical biology · 2025Article
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- Parametrically guided design of beta barrels and transmembrane nanopores using deep learning.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Computational design of potent and selective binders of BAK and BAX.Science advances · 2025Article
- Multistate and functional protein design using RoseTTAFold sequence space diffusion.Nature biotechnology · 2025Article
- Designed miniproteins potently inhibit and protect against MERS-CoV.Cell reports · 2025Article
- Computational design of serine hydrolases.Science (New York, N.Y.) · 2025Article
- One-Step Maleimide-Based Dual Functionalization of Protein N-Termini.Angewandte Chemie (International ed. in English) · 2025Article
- Parametrically guided design of beta barrels and transmembrane nanopores using deep learning.bioRxiv : the preprint server for biology · 2025Article
- Design and preparation of artificial heme-copper enzymes.Methods in enzymology · 2025Article
- Designed miniproteins potently inhibit and protect against MERS-CoV.bioRxiv : the preprint server for biology · 2024Article
- Computational design of serine hydrolases.bioRxiv : the preprint server for biology · 2024Article
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Authors and funding
6 authors.
Funding
Abstract
Site-specific protein cleavage is essential for many protein-production protocols and typically requires proteases. We report the development of a chemical protein-cleavage method that is achieved through the use of a sequence-specific nickel-assisted cleavage (SNAC)-tag. We demonstrate that the SNAC-tag can be inserted before both water-soluble and membrane proteins to achieve fusion protein cleavage under biocompatible conditions with efficiency comparable to that of enzymes, and that the method works even when enzymatic cleavages fail.
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