ArticleProceedings of the National Academy of Sciences of the United States of America2019
Multifunctionalized hydrogels foster hNSC maturation in 3D cultures and neural regeneration in spinal cord injuries.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers, 2 of them syntheses that pooled it.
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Who cites it
51 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- The Efficacy of Different Material Scaffold-Guided Cell Transplantation in the Treatment of Spinal Cord Injury in Rats: A Systematic Review and Network Meta-analysis.Cellular and molecular neurobiology · 2024Pooled it
- Combined application of neural stem/progenitor cells and scaffolds on locomotion recovery following spinal cord injury in rodents: a systematic review and meta-analysis.Neurosurgical review · 2022Pooled it
- Tumor Organoid and Microenvironment Cocultures: Implications for Basic and Translational Cancer Research.MedComm · 2026Review
- A Programmable, 3D Neuron-On-Chip Platform Integrating Near Real-Time Biosensing and Multiaxial Loading for Mechanobiological Injury Profiling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Emerging brain organoids: 3D models to decipher, identify and revolutionize brain.Bioactive materials · 2025Review
- Designing hydrogel for application in spinal surgery.Materials today. Bio · 2025Review
- Unveiling Interactions between Self-Assembling Peptides and Neuronal Membranes.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Regenerative medicine strategies for chronic complete spinal cord injury.Neural regeneration research · 2024Review
- Multiparametric in vitro and in vivo analysis of the safety profile of self-assembling peptides.Scientific reports · 2024Article
- Biomaterials and Cell Therapy Combination in Central Nervous System Treatments.ACS applied bio materials · 2024Review
- Restoring neuronal iron homeostasis revitalizes neurogenesis after spinal cord injury.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A 3D-Printed Dual Driving Forces Scaffold with Self-Promoted Cell Absorption for Spinal Cord Injury Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Recent Advances in the Development of Biomimetic Materials.Gels (Basel, Switzerland) · 2023Review
- Biomimetic Electrospun Self-Assembling Peptide Scaffolds for Neural Stem Cell Transplantation in Neural Tissue Engineering.Pharmaceutics · 2023Article
- Synthetic extracellular matrices with function-encoding peptides.Nature reviews bioengineering · 2023Review
- Loading neural stem cells on hydrogel scaffold improves cell retention rate and promotes functional recovery in traumatic brain injury.Materials today. Bio · 2023Article
- Generation of neural organoids for spinal-cord regeneration via the direct reprogramming of human astrocytes.Nature biomedical engineering · 2023Article
- Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels.Frontiers in bioengineering and biotechnology · 2023Article
- Therapeutic Effect of Biomimetic Scaffold Loaded with Human Amniotic Epithelial Cell-Derived Neural-like Cells for Spinal Cord Injury.Bioengineering (Basel, Switzerland) · 2022Article
- Biomaterials to enhance stem cell transplantation.Cell stem cell · 2022Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three-dimensional cell cultures are leading the way to the fabrication of tissue-like constructs useful to developmental biology and pharmaceutical screenings. However, their reproducibility and translational potential have been limited by biomaterial and culture media compositions, as well as cellular sources. We developed a construct comprising synthetic multifunctionalized hydrogels, serum-free media, and densely seeded good manufacturing practice protocol-grade human neural stem cells (hNSC). We tracked hNSC proliferation, differentiation, and maturation into GABAergic, glutamatergic, and cholinergic neurons, showing entangled electrically active neural networks. The neuroregenerative potential of the "engineered tissue" was assessed in spinal cord injuries, where hNSC-derived progenitors and predifferentiated hNSC progeny, embedded in multifunctionalized hydrogels, were implanted. All implants decreased astrogliosis and lowered the immune response, but scaffolds with predifferentiated hNSCs showed higher percentages of neuronal markers, better hNSC engraftment, and improved behavioral recovery. Our hNSC-construct enables the formation of 3D functional neuronal networks in vitro, allowing novel strategies for hNSC therapies in vivo.
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