Evidence map›Paper›PMID 30922402›Full record

ArticleMolecular cancer2019

Circular RNA circ-DONSON facilitates gastric cancer growth and invasion via NURF complex dependent activation of transcription factor SOX4.

Lixian Ding, Yuying Zhao, Shuwei Dang, Yue Wang, Xinglong Li, Xiaotong Yu, Zhongsheng Li, Jiufeng Wei, Ming Liu, Guodong Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cancer, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 154 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
154citing papers in PubMed, 1 pooled it
22.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

154 citing papers in PubMed, 1 synthesis or guideline pooled it, 365 citations in OpenAlex.

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  20. Circ_0008035 Promotes Gastric Cancer Development via the miR-429/SMAD2 Cascade.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2024
    Article

94 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Lixian DingDepartment of General Surgery, the Fourth Affiliated Hospital of Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, People's Republic of China.
Yuying ZhaoDepartment of Medical Oncology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150001, People's Republic of China.
Shuwei DangDepartment of General Surgery, the Fourth Affiliated Hospital of Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, People's Republic of China.
Yue WangDepartment of Pharmacology and Toxicology, Wright State University, Fairborn, OH, 45435, USA.
Xinglong LiDepartment of General Surgery, the Fourth Affiliated Hospital of Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, People's Republic of China.
Xiaotong YuDepartment of General Surgery, the Fourth Affiliated Hospital of Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, People's Republic of China.
Zhongsheng LiDepartment of General Surgery, the Fourth Affiliated Hospital of Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, People's Republic of China.
Jiufeng WeiDepartment of General Surgery, the Fourth Affiliated Hospital of Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, People's Republic of China.
Ming LiuDepartment of General Surgery, the Fourth Affiliated Hospital of Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, People's Republic of China.
Guodong LiDepartment of General Surgery, the Fourth Affiliated Hospital of Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, People's Republic of China. liguodong@ems.hrbmu.edu.cn.ORCID 0000-0003-0526-3427
Harbin Medical University · CNWright State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCircular RNAs (circRNAs) are a novel type of noncoding RNAs and play important roles in tumorigenesis, including gastric cancer (GC). However, the functions of most circRNAs remain poorly understood. In our study, we aimed to investigate the functions of a new circRNA circ-DONSON in GC progression.

methodsThe expression of circ-DONSON in gastric cancer tissues and adjacent normal tissues was analyzed by bioinformatics method, qRT-PCR, Northern blotting and in situ hybridization (ISH). The effects of circ-DONSON on GC cell proliferation, apoptosis, migration and invasion were measured by using CCK8, colony formation, EdU, immunofluorescence (IF), FACS and Transwell assays. qRT-PCR and Western blotting were utilized to validate how circ-DONSON regulates SOX4 expression. ChIP, DNA fluorescence in situ hybridization (DNA-FISH) and DNA accessibility assays were used to investigate how circ-DONSON regulates SOX4 transcription. The interaction between circ-DONSON and NURF complex was evaluated by mass spectrum, RNA immunoprecipitation (RIP), pulldown and EMSA assays. Xenograft mouse model was used to analyze the effect of circ-DONSON on GC growth in vivo.

resultsElevated expression of circ-DONSON was observed in GC tissues and positively associated with advanced TNM stage and unfavorable prognosis. Silencing of circ-DONSON significantly suppressed the proliferation, migration and invasion of GC cells while promoting apoptosis. circ-DONSON was localized in the nucleus, recruited the NURF complex to SOX4 promoter and initiated its transcription. Silencing of the NURF complex subunit SNF2L, BPTF or RBBP4 similarly attenuated GC cell growth and increased apoptosis. circ-DONSON knockdown inhibited GC growth in vivo.

conclusioncirc-DONSON promotes GC progression through recruiting the NURF complex to initiate SOX4 expression.

Indexed as

AnimalsAntigens, NuclearApoptosisBiomarkers, TumorBromodomain Containing ProteinsCell Cycle ProteinsCell MovementCell ProliferationDisease ProgressionDNA-Binding ProteinsFemaleFollow-Up StudiesGene Expression Regulation, NeoplasticHumansMiceMice, Inbred BALB CAntigens, NuclearBiomarkers, TumorBromodomain Containing ProteinsCell Cycle ProteinsDNA-Binding ProteinsDONSON protein, humanfetal Alzheimer antigenNerve Tissue ProteinsNuclear ProteinsRBBP4 protein, humanRetinoblastoma-Binding Protein 4RNARNA, CircularSMARCA1 protein, humanSOX4 protein, humanSOXC Transcription FactorsTranscription Factorscirc-DONSONGastric cancerNURFProgressionSOX4

Identifiers

PMID30922402
PMCPMC6437893
OpenAlexW2934231425

What OpenQuestion holds

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LicenceCC BY
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.