Evidence map›Paper›PMID 30922239›Full record

ArticleBMC plant biology2019

Structure and function of class III pistil-specific extensin-like protein in interspecific reproductive barriers.

Camila M L Alves, Andrzej K Noyszewski, Alan G Smith

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Camila M L AlvesDepartment of Horticultural Science, University of Minnesota, Saint Paul, MN, 55108, USA.
Andrzej K NoyszewskiDepartment of Horticultural Science, University of Minnesota, Saint Paul, MN, 55108, USA.
Alan G SmithDepartment of Horticultural Science, University of Minnesota, Saint Paul, MN, 55108, USA. smith022@umn.edu.ORCID http://orcid.org/0000-0003-4969-5467
University of Minnesota · US

Funding

Applied Plant Science Metric Fellowship not applicableCoordenação de Aperfeicoamento de Pessoal de Nível Superior - CAPES (Science without Borders) not applicableUniversity of Minnesota, Agricultural Experiment Station MIN-21-064University of Minnesota, Alexander and Lydia Anderson not applicableUniversity of Minnesota, Bridging funds not applicable
6 · The paper itself

Abstract

backgroundThe transmitting tissue of the style is the pathway for pollen tube growth to the ovules and has components that function in recognizing and discriminating appropriate pollen genotypes. In Nicotiana tabacum, the class III pistil extensin-like (PELPIII) arabinogalactan protein is essential for the inhibition of N. obtusifolia pollen tube growth. The transmitting tissue-specific (TTS) arabinogalactan protein amino acid sequence and expression pattern is similar to PELPIII, but it facilitates self-pollinated N. tabacum. The TTS and PELPIII arabinogalactan protein can be divided into the less conserved N-terminal (NTD) and the more conserved C-terminal (CTD) domains. This research tested whether the NTD is the key domain in determining PELPIII function in the inhibition of interspecific pollen tube growth. Three variant PELPIII gene constructs were produced where the PELPIII NTD was exchanged with the TTS NTD and a single amino acid change (cysteine to alanine) was introduced into the PELPIII NTD. The PELPIII variants of N. tabacum were tested for activity by measuring the inhibition N. obtusifolia pollen tube growth by using them to complement a 3'UTR RNAi transgenic line with reduced PELPIII mRNA.

resultsThe RNAi N. tabacum line had reduced PELPIII mRNA accumulation and reduced inhibition of N. obtusifolia pollen tube growth, but had no effect on self-pollen tube growth or pollen tube growth of 12 other Nicotiana species. The NTD of PELPIII with either the PELPIII or TTS CTDs complemented the loss PELPIII activity in the RNAi transgenic line as measured by inhibition of N. obtusifolia pollen tube growth. The TTS NTD with the PELPIII CTD and a variant PELPIII with a cysteine to alanine mutation in its NTD failed to complement the loss of PELPIII activity and did not inhibit N. obtusifolia pollen tube growth.

conclusionThe NTD is a key determinant in PELPIII's function in regulating interspecific pollen tube growth and is a first step toward understanding the mechanism of how PELPIII NTD regulates pollen tube growth.

Indexed as

3' Untranslated RegionsGene Expression Regulation, PlantNicotianaPlant ProteinsPlants, Genetically ModifiedPollen TubeProtein DomainsReproductionRNA Interference3' Untranslated RegionsPlant Proteins3’UTR RNAiArabinogalactan proteinsGene complementationNicotiana obtusifoliaNicotiana tabacumPELPIIIPollen tube growthTTS

Identifiers

PMID30922239
PMCPMC6440088
OpenAlexW2942729632

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.