ArticlePLoS pathogens2019
Tenuivirus utilizes its glycoprotein as a helper component to overcome insect midgut barriers for its circulative and propagative transmission.
Article in PLoS pathogens, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
- Construction and Biological Characterization of Papaya Leaf Curl Guangdong Virus Infectious cDNA Clone.Microbial biotechnology · 2026Article
- Purα is an essential host factor for rice stripe virus accumulation and transmission in the small brown planthopper.Archives of virology · 2026Article
- Tetraspanin SfCD9 as a Key Membrane Binding Factor of SRBSDV P10 Facilitates Viral Entry Into Sogatella furcifera Midgut Epithelial Cells via Clathrin-Mediated Endocytosis.Molecular plant pathology · 2025Article
- Virus Isolation and Rice Protoplast Infection.Bio-protocol · 2025Article
- A plant virus attenuates the Toll immune pathway by degradation of Pellino to facilitate viral infection in insect vectors.Journal of virology · 2025Article
- Revealing the role of theFrontiers in plant science · 2025Article
- Article
- The JAK-STAT pathway promotes persistent viral infection by activating apoptosis in insect vectors.PLoS pathogens · 2023Article
- Determinants of Virus Variation, Evolution, and Host Adaptation.Pathogens (Basel, Switzerland) · 2022Review
- Nonconcomitant host-to-host transmission of multipartite virus genome segments may lead to complete genome reconstitution.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Flotillin 2 Facilitates the Infection of a Plant Virus in the Gut of Insect Vector.Journal of virology · 2022Article
- Heat shock cognate protein 70 is required for rice stripe tenuivirus accumulation and transmission in small brown planthopper.Archives of virology · 2022Article
- Insights Into the Effect of Rice Stripe Virus P2 on Rice Defense by Comparative Proteomic Analysis.Frontiers in microbiology · 2022Article
- Membrane association of importin α facilitates viral entry into salivary gland cells of vector insects.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Development of a Mini-Replicon-Based Reverse-Genetics System for Rice Stripe Tenuivirus.Journal of virology · 2021Article
- Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector.Viruses · 2021Article
- Article
- The Bunyavirales: The Plant-Infecting Counterparts.Viruses · 2021Review
- A class of independently evolved transcriptional repressors in plant RNA viruses facilitates viral infection and vector feeding.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Development of Rice Stripe Tenuivirus Minireplicon Reverse Genetics Systems Suitable for Analyses of Viral Replication and Intercellular Movement.Frontiers in microbiology · 2021Article
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many persistent transmitted plant viruses, including rice stripe virus (RSV), cause serious damage to crop production worldwide. Although many reports have indicated that a successful insect-mediated virus transmission depends on a proper interaction between the virus and its insect vector, the mechanism(s) controlling this interaction remained poorly understood. In this study, we used RSV and its small brown planthopper (SBPH) vector as a working model to elucidate the molecular mechanisms underlying the entrance of RSV virions into SBPH midgut cells for virus circulative and propagative transmission. We have determined that this non-enveloped tenuivirus uses its non-structural glycoprotein NSvc2 as a helper component to overcome the midgut barrier(s) for RSV replication and transmission. In the absence of this glycoprotein, purified RSV virions were unable to enter SBPH midgut cells. In the RSV-infected cells, this glycoprotein was processed into two mature proteins: an amino-terminal protein (NSvc2-N) and a carboxyl-terminal protein (NSvc2-C). Both NSvc2-N and NSvc2-C interact with RSV virions. Our results showed that the NSvc2-N could bind directly to the surface of midgut lumen via its N-glycosylation sites. Upon recognition, the midgut cells underwent endocytosis followed by compartmentalization of RSV virions and NSvc2 into early and then late endosomes. The NSvc2-C triggered cell membrane fusion via its highly conserved fusion loop motifs under the acidic condition inside the late endosomes, leading to the release of RSV virions from endosomes into cytosol. In summary, our results showed for the first time that a rice tenuivirus utilized its glycoprotein NSvc2 as a helper component to ensure a proper interaction between its virions and SBPH midgut cells for its circulative and propagative transmission.
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