ArticleEuropean biophysics journal : EBJ2019
Low cost non-electromechanical technique for the purification of giant unilamellar vesicles.
Article in European biophysics journal : EBJ, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Electric field-induced rupture kinetics of giant unilamellar vesicles with varying gramicidin A content in membrane.PloS one · 2026Article
- Electroporation in lipid vesicles by varying PEG-grafted lipids in their membranes: an experimental and simulation study.RSC advances · 2025Article
- Interaction of anionic FeRSC advances · 2024Review
- Probability and kinetics of rupture and electrofusion in giant unilamellar vesicles under various frequencies of direct current pulses.PloS one · 2024Article
- Article
- Effects of cholesterol on the anionic magnetite nanoparticle-induced deformation and poration of giant lipid vesicles.RSC advances · 2022Article
- Effects of sugar concentration on the electroporation, size distribution and average size of charged giant unilamellar vesicles.European biophysics journal : EBJ · 2022Article
- Identifying and Manipulating Giant Vesicles: Review of Recent Approaches.Micromachines · 2022Review
- Influence of sugar concentration on the vesicle compactness, deformation and membrane poration induced by anionic nanoparticles.PloS one · 2022Article
- Article
- Effects of cholesterol on the size distribution and bending modulus of lipid vesicles.PloS one · 2022Article
- Recent developments in the kinetics of ruptures of giant vesicles under constant tension.RSC advances · 2021Review
- Analysis of purification of charged giant vesicles in a buffer using their size distribution.The European physical journal. E, Soft matter · 2021Article
- Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.PloS one · 2021Article
- A new purification technique to obtain specific size distribution of giant lipid vesicles using dual filtration.PloS one · 2021Article
- An investigation into the critical tension of electroporation in anionic lipid vesicles.European biophysics journal : EBJ · 2021Article
- Location of Peptide-Induced Submicron Discontinuities in the Membranes of Vesicles Using ImageJ.Journal of fluorescence · 2020Article
- Influence of cholesterol on electroporation in lipid membranes of giant vesicles.European biophysics journal : EBJ · 2020Article
- Kinetics of irreversible pore formation under constant electrical tension in giant unilamellar vesicles.European biophysics journal : EBJ · 2020Article
- Study of molecular transport through a single nanopore in the membrane of a giant unilamellar vesicle using COMSOL simulation.European biophysics journal : EBJ · 2020Article
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Authors and funding
6 authors.
Funding
Abstract
Lipid membranes of giant unilamellar vesicles (GUVs) with diameters greater than 10 μm are promising model membranes for investigating the physical and biological properties of the biomembranes of cells. These are extensively used for the study of the interaction of various membrane-active agents, where purified and similar-size oil-free GUVs are necessary. Although the existing membrane filtering method provides the required quality and quantity of GUVs, it includes a relatively expensive double-headed peristaltic pump. In our proposed non-electromechanical technique, gravity is used to maintain the flow of buffer, wherein the flow rate of buffer with the suspension of GUVs is controlled by a locally available low cost roller clamp regulator. We have characterized the results of this non-electromechanical approach in terms of size distribution, average size, flow rate and efficiency for dioleoylphosphatidylglycerol (DOPG)/dioleoylphosphatidylcholine (DOPC)-GUVs prepared by the natural swelling method. The technique purifies the GUVs by removing the non-entrapped solutes at an optimum flow rate 1.0-2.0 mL/min. In addition, it gives similar results to the pump-driven membrane filtering method. Therefore, it might be a cost effective technique for the purification of GUVs without employing any electromechanical devices.
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Registered trials
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