Evidence map›Paper›PMID 30918467›Full record

ArticleMediators of inflammation2019

Cytokines and Chemokines in Pediatric Appendicitis: A Multiplex Analysis of Inflammatory Protein Mediators.

S Ali Naqvi, Graham C Thompson, Ari R Joffe, Jaime Blackwood, Dori-Ann Martin, Mary Brindle, Herman W Barkema, Craig N Jenne

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mediators of inflammation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
3.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

S Ali NaqviDepartment of Community Health Sciences, Cumming School of Medicine, University of Calgary, Calgary AB, Canada.ORCID 0000-0002-1760-958X
Graham C ThompsonDepartment of Pediatrics, Cumming School of Medicine, University of Calgary, Calgary AB, Canada.ORCID 0000-0002-6781-4186
Ari R JoffeDepartment of Pediatrics, Division of Critical Care, University of Alberta, Edmonton AB, Canada.
Jaime BlackwoodDepartment of Pediatrics, Division of Critical Care Medicine, Cumming School of Medicine, University of Calgary, Calgary AB, Canada.
Dori-Ann MartinDepartment of Pediatrics, Division of Critical Care Medicine, Cumming School of Medicine, University of Calgary, Calgary AB, Canada.ORCID 0000-0002-8870-7873
Mary BrindleDepartment of Surgery, Cumming School of Medicine, University of Calgary, Calgary AB, Canada.
Herman W BarkemaDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary AB, Canada.
Craig N JenneDepartment of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, Calgary AB, Canada.ORCID 0000-0001-8637-0181
University of Calgary · CAUniversity of Alberta · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesWe aimed to demonstrate the potential of precision medicine to describe the inflammatory landscape present in children with suspected appendicitis. Our primary objective was to determine levels of seven inflammatory protein mediators previously associated with intra-abdominal inflammation (C-reactive protein-CRP, procalcitonin-PCT, interleukin-6 (IL), IL-8, IL-10, monocyte chemoattractant protein-1-MCP-1, and serum amyloid A-SAA) in a cohort of children with suspected appendicitis. Subsequently, using a multiplex proteomics approach, we examined an expansive array of novel candidate cytokine and chemokines within this population.

methodsWe performed a secondary analysis of targeted proteomics data from Alberta Sepsis Network studies. Plasma mediator levels, analyzed by Luminex multiplex assays, were evaluated in children aged 5-17 years with nonappendicitis abdominal pain (NAAP), acute appendicitis (AA), and nonappendicitis sepsis (NAS). We used multivariate regression analysis to evaluate the seven target proteins, followed by decision tree and heat mapping analyses for all proteins evaluated.

results185 children were included: 83 with NAAP, 79 AA, and 23 NAS. Plasma levels of IL-6, CRP, MCP-1, PCT, and SAA were significantly different in children with AA compared to those with NAAP (

conclusionsMultiplex proteomic analyses described the inflammatory landscape of children presenting to the ED with suspected appendicitis. We have demonstrated the feasibility of this approach to identify potential novel candidate cytokines/chemokine patterns associated with a specific illness (appendicitis) amongst those with a broad ED presentation (abdominal pain). This approach can be modelled for future research initiatives in pediatric emergency medicine.

Indexed as

AdolescentAppendicitisChemokine CCL2ChemokinesChildChild, PreschoolCytokinesHumansInterleukin-10Interleukin-6Interleukin-8Precision MedicineProspective StudiesProteomicsSepsisSerum Amyloid A ProteinCCL2 protein, humanChemokine CCL2ChemokinesCytokinesInterleukin-10Interleukin-6Interleukin-8Serum Amyloid A Protein

Identifiers

PMID30918467
PMCPMC6409077
OpenAlexW2916218940

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.