ArticleScientific reports2019
Improving eDNA yield and inhibitor reduction through increased water volumes and multi-filter isolation techniques.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Optimizing Marine eDNA Protocols for Rare Megafauna: A Case Study on Dugongs in the Western Indian Ocean.Ecology and evolution · 2026Article
- Development and field evaluation of a multiplex qPCR assay for environmental DNA detection of Schistosoma mekongi and its intermediate snail host Neotricula aperta in the Mekong River Basin.Infectious diseases of poverty · 2026Article
- Environmental DNA Metabarcoding Effectively Detects Invasive Species, Pests, and Community Changes in Taiwan's Rice Fields.Ecology and evolution · 2026Article
- Recovering Historical eDNA From Museum-Preserved Filter Feeders via Non-Destructive Metabarcoding.Molecular ecology resources · 2026Article
- Molecular identification of pelagic fish eggs with oil globule.Molecular biology reports · 2026Article
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- STR analysis of human DNA recovered from bathwater and other water samples for forensic identification.PloS one · 2026Article
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- Integrating molecular methods and biophysical modeling to assess functional connectivity between marine protected areas.Ecological applications : a publication of the Ecological Society of America · 2025Article
- An eDNA Survey of Plant Biodiversity in a Local Dam Within South Africa's Largest City.Ecology and evolution · 2025Article
- Counting soil microbial communities: the impact of qPCR platform and mastermix on accuracy and precision.FEMS microbiology ecology · 2025Article
- An optimized eDNA protocol for fish tracking in estuarine environments.Scientific reports · 2025Article
- Article
- Molecular Identification of Human and Plant Pathogens in Municipal Domestic Wastewater for Hydroponic System Applications.International journal of microbiology · 2025Article
- No pump, no problem: evaluating passive eDNA sampling for marine biomonitoring of a nuisance macroalga.PeerJ · 2025Article
- Environmental DNA-Based Identification of Non-Native Fish in Beijing: Diversity, Geographical Distribution, and Interactions with Native Taxa.Animals : an open access journal from MDPI · 2024Article
- Improving the efficiency of adaptive management methods in multiple fishways using environmental DNA.PloS one · 2024Article
- Article
- Review
- Methodological considerations for aqueous environmental RNA collection, preservation, and extraction.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
To inform management and conservation decisions, environmental DNA (eDNA) methods are used to detect genetic material shed into the water by imperiled and invasive species. Methodological enhancements are needed to reduce filter clogging, PCR inhibition, and false-negative detections when eDNA is at low concentrations. In the first of three simple experiments, we sought to ameliorate filter clogging from particulates and organic material through a scaled-up, multi-filter protocol. We combined four filters in a 5 mL Phenol-Chloroform-Isoamyl (PCI) procedure to allow for larger volumes of water (~1 L) to be filtered rapidly. Increasing the filtered water volume by four times resulted in 4.4X the yield of target DNA. Next, inhibition from organic material can reduce or block eDNA detections in PCR-based assays. To remove inhibitory compounds retained during eDNA isolation, we tested three methods to chemically strip inhibitors from eDNA molecules. The use of CTAB as a short-term (5-8 day) storage buffer, followed by a PCI isolation, resulted in the highest eDNA yields. Finally, as opposed to a linear relationship among increasing concentrations of filtered genomic eDNA, we observed a sharp change between the lower (70-280 ng) and higher (420-560 ng) amounts. This may be important for effectively precipitating eDNA during protocol testing.
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