Evidence map›Paper›PMID 30918257›Full record

ArticleNature communications2019

Directing curli polymerization with DNA origami nucleators.

Xiuhai Mao, Ke Li, Mengmeng Liu, Xinyu Wang, Tianxin Zhao, Bolin An, Mengkui Cui, Yingfeng Li, Jiahua Pu, Jiang Li and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

Xiuhai MaoMaterials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.ORCID http://orcid.org/0000-0002-4039-8733
Ke LiMaterials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Mengmeng LiuShanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
Xinyu WangMaterials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Tianxin ZhaoMaterials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Bolin AnMaterials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Mengkui CuiMaterials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Yingfeng LiMaterials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Jiahua PuMaterials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Jiang LiDivision of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
Lihua WangDivision of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
Timothy K LuSynthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Chunhai FanSchool of Chemistry and Chemical Engineering, and Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China. fanchunhai@sjtu.edu.cn.ORCID http://orcid.org/0000-0002-7171-7338
Chao ZhongMaterials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China. zhongchao@shanghaitech.edu.cn.ORCID http://orcid.org/0000-0002-6638-3652
ShanghaiTech University · CNChinese Academy of Sciences · CNShanghai Jiao Tong University · CNEast China Normal University · CNMassachusetts Institute of Technology · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The physiological or pathological formation of fibrils often relies on molecular-scale nucleators that finely control the kinetics and structural features. However, mechanistic understanding of how protein nucleators mediate fibril formation in cells remains elusive. Here, we develop a CsgB-decorated DNA origami (CB-origami) to mimic protein nucleators in Escherichia coli biofilm that direct curli polymerization. We show that CB-origami directs curli subunit CsgA monomers to form oligomers and then accelerates fibril formation by increasing the proliferation rate of primary pathways. Fibrils grow either out from (departure mode) or towards the nucleators (arrival mode), implying two distinct roles of CsgB: as nucleation sites and as trap sites to capture growing nanofibrils in vicinity. Curli polymerization follows typical stop-and-go dynamics but exhibits a higher instantaneous elongation rate compared with independent fibril growth. This origami nucleator thus provides an in vitro platform for mechanistically probing molecular nucleation and controlling directional fibril polymerization for bionanotechnology.

Indexed as

PolymerizationBiofilmsDNAEscherichia coliEscherichia coli ProteinsKineticscsgA protein, E coliCsgB protein, E coliDNAEscherichia coli Proteins

Identifiers

PMID30918257
PMCPMC6437208
OpenAlexW2923165027

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.