ArticleBMC research notes2019
Mutagenesis of seed storage protein genes in Soybean using CRISPR/Cas9.
Article in BMC research notes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 80 citations in OpenAlex.
- CRISPR/Cas9 editing of β-Conglycinin subunits reduces IgE binding in soybean [Glycine max (L.) Merr.].BMC plant biology · 2026Article
- Advances in gene editing for legume improvement: technologies, progress, and prospects.Frontiers in genome editing · 2026Review
- Targets and strategies to design soybean seed composition traits.The plant genome · 2025Review
- CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks.Journal of advanced research · 2025Review
- QTL mapping and multi-omics identify candidate genes for protein and oil in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Simultaneous site-directed mutagenesis for soybean ß-amyrin synthase genes via DNA-free CRISPR/Cas9 system using a single gRNA.Plant cell reports · 2025Article
- Genome editing in grain legumes for food security.Frontiers in genome editing · 2025Review
- Genome editing of an oxalyl-CoA synthetase gene in Lathyrus sativus reveals its role in oxalate metabolism.Plant cell reports · 2024Article
- Multi-Omics Analysis of a Chromosome Segment Substitution Line Reveals a New Regulation Network for Soybean Seed Storage Profile.International journal of molecular sciences · 2024Article
- Genetic mapping and functional genomics of soybean seed protein.Molecular breeding : new strategies in plant improvement · 2023Article
- Review
- Article
- Insights into mechanisms of seed longevity in soybean: a review.Frontiers in plant science · 2023Review
- Advances in CRISPR/Cas9-based research related to soybean [Frontiers in plant science · 2023Review
- Soybean genetic resources contributing to sustainable protein production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Review
- Efficient gene targeting in soybean using Ochrobactrum haywardense-mediated delivery of a marker-free donor template.Plant physiology · 2022Article
- An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes.BMC biotechnology · 2022Article
- Progress in soybean functional genomics over the past decade.Plant biotechnology journal · 2022Review
- Gene-Editing Technologies and Applications in Legumes: Progress, Evolution, and Future Prospects.Frontiers in genetics · 2022Review
- Progress in Soybean Genetic Transformation Over the Last Decade.Frontiers in plant science · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
objectiveSoybean seeds are an important source of vegetable proteins for both food and industry worldwide. Conglycinins (7S) and glycinins (11S), which are two major families of storage proteins encoded by a small family of genes, account for about 70% of total soy seed protein. Mutant alleles of these genes are often necessary in certain breeding programs, as the relative abundance of these protein subunits affect amino acid composition and soy food properties. In this study, we set out to test the efficiency of the CRISPR/Cas9 system in editing soybean storage protein genes using Agrobacterium rhizogenes-mediated hairy root transformation system.
resultsWe designed and tested sgRNAs to target nine different major storage protein genes and detected DNA mutations in three storage protein genes in soybean hairy roots, at a ratio ranging from 3.8 to 43.7%. Our work provides a useful resource for future soybean breeders to engineer/develop varieties with mutations in seed storage proteins.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.