ArticleMolecules (Basel, Switzerland)2019
The Quality of DNA Isolated from Processed Food and Feed via Different Extraction Procedures.
Article in Molecules (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 75 citations in OpenAlex.
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- Parvalbumin Gene: A Valuable Marker for Pike Authentication and Allergen Risk Assessment.Journal of agricultural and food chemistry · 2024Article
- Enhancing the authenticity of animal by-products: harmonization of DNA extraction methods from novel ingredients.Frontiers in chemistry · 2024Article
- Article
- Development of a tetra-primer ARMS-PCR for identification of sika and red deer and their hybrids.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- Propidium Monoazide-Treated, Cell-Direct, Quantitative PCR for Detecting Viable Chloramphenicol-ResistantGenes · 2023Article
- An efficient, fast and inexpensive method for genomic DNA extraction of fish tissue.Molecular biology reports · 2023Article
- Proficiency of European GMO control laboratories to quantify MON89788 soybean in a meat pâté matrix.Food control · 2023Article
- Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for the Rapid Authentication of Three Swimming Crab Species.Foods (Basel, Switzerland) · 2022Article
- Application of microbial analyses to feeds and potential implications for poultry nutrition.Poultry science · 2022Review
- Combination and improvement of conventional DNA extraction methods in Actinobacteria to obtain high-quantity and high-quality DNA.Iranian journal of microbiology · 2022Article
- Mutations in TP53 and PIK3CA genes in hepatocellular carcinoma patients are associated with chronic Schistosomiasis.Saudi journal of biological sciences · 2022Article
- Determining the presence of undeclared animal species using Real-time PCR in canned and ready-to-eat meat products in South Africa.Journal of food science and technology · 2021Article
- Identification of Selected Tuna Species in Commercial Products.Molecules (Basel, Switzerland) · 2021Article
- A Practical Approach to Identifying Processed White Meat of Guinea Fowl, Rabbit, and Selected Fish Species Using End-Point PCR.International journal of food science · 2021Article
- Cyprus Sausages' Bacterial Community Identification Through Metataxonomic Sequencing: Evaluation of the Impact of Different DNA Extraction Protocols on the Sausages' Microbial Diversity Representation.Frontiers in microbiology · 2021Article
- NGS-based barcoding with mini-COI gene target is useful for pet food market surveys aimed at mislabelling detection.Scientific reports · 2020Article
- Procedures for DNA Extraction from Opium Poppy (Foods (Basel, Switzerland) · 2020Article
- Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation.Royal Society open science · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The extraction of DNA is a critical step for species identification by PCR analysis in processed food and feed products. In this study, eight DNA extraction procedures were compared-DNeasy Blood and Tissue Kit, DNeasy mericon Food Kit, chemagic DNA Tissue 10 Kit, Food DNA Isolation Kit, UltraPrep Genomic DNA Food Mini Prep Kit, High Pure PCR Template Preparation Kit, phenol-chloroform extraction, and NucleoSpin Food-Using self-prepared samples from both raw and heat-processed and/or mechanically treated muscles and different types of meat products and pet food (pork, beef, and chicken). The yield, purity, and suitability of DNA for PCR amplification was evaluated. Additionally, comparisons between the effectiveness of various extraction methods were made with regard to price, and labor- and time-intensiveness. It was found that the DNeasy mericon Food Kit was the optimal choice for the extraction of DNA from raw muscle, heat-treated muscle, and homemade meat products from multiple and single species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.