ArticlePloS one2019
Osmotic stress activates nif and fix genes and induces the Rhizobium tropici CIAT 899 Nod factor production via NodD2 by up-regulation of the nodA2 operon and the nodA3 gene.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 25 citations in OpenAlex.
- Response of nitrogen-fixing plant symbioses to changing temperature.Journal of integrative plant biology · 2026Review
- Genetic Characterization and Symbiotic Performance of Soybean Rhizobia Under Cold and Water-Deficient Conditions in Poland.Plants (Basel, Switzerland) · 2025Article
- Nod Factor Lipopolysaccharide Purification to Study Nitrogen-Fixing Bacteria Symbiosis with Legumes.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Ionomic and proteomic changes highlight the effect of silicon supply on the nodules functioning ofFrontiers in plant science · 2024Article
- Isoflavonoid metabolism in leguminous plants: an update and perspectives.Frontiers in plant science · 2024Review
- Marker trait association for biological nitrogen fixation traits in an interspecific cross of chickpea (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Non-Ionic Osmotic Stress Induces the Biosynthesis of Nodulation Factors and Affects Other Symbiotic Traits inBiology · 2023Article
- Nod factor perception: an integrative view of molecular communication during legume symbiosis.Plant molecular biology · 2022Review
- The Rhizobium tropici CIAT 899 NodD2 protein promotes symbiosis and extends rhizobial nodulation range by constitutive nodulation factor synthesis.Journal of experimental botany · 2022Article
- The Rhizobial Type 3 Secretion System: The Dr. Jekyll and Mr. Hyde in the Rhizobium-Legume Symbiosis.International journal of molecular sciences · 2022Review
- Utilization of Legume-Nodule Bacterial Symbiosis in Phytoremediation of Heavy Metal-Contaminated Soils.Biology · 2022Review
- A Perspective on Developing a Plant 'Holobiont' for Future Saline Agriculture.Frontiers in microbiology · 2022Review
- Phenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction.Scientific reports · 2021Article
- The Rhizobium-Legume Symbiosis: Co-opting Successful Stress Management.Frontiers in plant science · 2021Review
- Metabolic Engineering of Isoflavones: An Updated Overview.Frontiers in plant science · 2021Review
- OnfD, an AraC-Type Transcriptional Regulator Encoded byApplied and environmental microbiology · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The symbiosis between rhizobia and legumes is characterized by a complex molecular dialogue in which the bacterial NodD protein plays a major role due to its capacity to activate the expression of the nodulation genes in the presence of appropiate flavonoids. These genes are involved in the synthesis of molecules, the nodulation factors (NF), responsible for launching the nodulation process. Rhizobium tropici CIAT 899, a rhizobial strain that nodulates Phaseolus vulgaris, is characterized by its tolerance to multiple environmental stresses such as high temperatures, acidity or elevated osmolarity. This strain produces nodulation factors under saline stress and the same set of CIAT 899 nodulation genes activated by inducing flavonoids are also up-regulated in a process controlled by the NodD2 protein. In this paper, we have studied the effect of osmotic stress (high mannitol concentrations) on the R. tropici CIAT 899 transcriptomic response. In the same manner as with saline stress, the osmotic stress mediated NF production and export was controlled directly by NodD2. In contrast to previous reports, the nodA2FE operon and the nodA3 and nodD1 genes were up-regulated with mannitol, which correlated with an increase in the production of biologically active NF. Interestingly, in these conditions, this regulatory protein controlled not only the expression of nodulation genes but also the expression of other genes involved in protein folding and synthesis, motility, synthesis of polysaccharides and, surprinsingly, nitrogen fixation. Moreover, the non-metabolizable sugar dulcitol was also able to induce the NF production and the activation of nod genes in CIAT 899.
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