ArticleLangmuir : the ACS journal of surfaces and colloids2019
Super-Resolution Fluorescence Microscopy Study of the Production of K1 Capsules by Escherichia coli: Evidence for the Differential Distribution of the Capsule at the Poles and the Equator of the Cell.
Article in Langmuir : the ACS journal of surfaces and colloids, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Modeling Multiscale Architecture of Biofilm Extracellular Matrix and Its Role in Oxygen Transport.Biotechnology and bioengineering · 2026Article
- Evaluation of bedding treatments to inhibit Klebsiella pneumoniae growth in wood shavings and sand.JDS communications · 2026Article
- Effect of biofilm lifestyle caused by water matric potential on invasion of exogenous plasmid.ISME communications · 2026Article
- Mechanisms governing bacterial capsular polysaccharide attachment and chain length.Annals of the New York Academy of Sciences · 2025Review
- Targeting the Weak Spot: Preferential Disruption of Bacterial Poles by Janus Nanoparticles.Nano letters · 2024Article
- Rhizosphere bacterial exopolysaccharides: composition, biosynthesis, and their potential applications.Archives of microbiology · 2024Review
- Synergy between Group 2 capsules and lipopolysaccharide underpins serum resistance in extra-intestinal pathogenicMicrobiology (Reading, England) · 2024Article
- Article
- Surveying membrane landscapes: a new look at the bacterial cell surface.Nature reviews. Microbiology · 2023Review
- A multi-enzyme machine polymerizes the Haemophilus influenzae type b capsule.Nature chemical biology · 2023Article
- Modeling and Simulation of Bacterial Outer Membranes with Lipopolysaccharides and Capsular Polysaccharides.Journal of chemical information and modeling · 2023Article
- Genetic determinants of host tropism in Klebsiella phages.Cell reports · 2023Article
- A bioorthogonal chemistry approach to detect the K1 polysialic acid capsule inRSC chemical biology · 2023Article
- Article
- Nutrient conditions are primary drivers of bacterial capsule maintenance inProceedings. Biological sciences · 2021Article
- Modular prophage interactions driven by capsule serotype select for capsule loss under phage predation.The ISME journal · 2020Article
- Deciphering the role of the capsule of Klebsiella pneumoniae during pathogenesis: A cautionary tale.Molecular microbiology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The production of Escherichia coli K1 serotype capsule was investigated using direct stochastic optical reconstruction microscopy with live bacteria and graphene oxide-coated coverslips, overcoming many morphological artifacts found in other high-resolution imaging techniques. Super-resolution fluorescence images showed that the K1 capsular polysaccharide is not uniformly distributed on the cell surface, as previously thought. These studies demonstrated that on the cell surfaces the K1 capsule at the poles had bimodal thicknesses of 238 ± 41 and 323 ± 62 nm, whereas at the equator, there was a monomodal thickness of 217 ± 29 nm. This bimodal variation was also observed in high-pressure light-scattering chromatography measurements of purified K1 capsular polysaccharide. Particle tracking demonstrated that the formation of the capsule was dominated by the expansion of lyso-phosphatidylglycerol (lyso-PG) rafts that anchor the capsular polysaccharide in the outer membrane, and the expansion of these rafts across the cell surface was driven by new material transported through the capsular biosynthesis channels. The discovery of thicker capsules at the poles of the cell will have implications in mediating interactions between the bacterium and its immediate environment.
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