Evidence map›Paper›PMID 30916220›Full record

ArticleBrazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica2019

Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method.

Chao Li, Gongyu Fu, Yaoqiang Shi, A-Mei Zhang, Xueshan Xia, Yue Fang, Xiaoqin Mao, Jie Jiang, Yuzhu Song, Guangying Yang

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Chao LiFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.ORCID http://orcid.org/0000-0003-4240-1382
Gongyu FuFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.ORCID http://orcid.org/0000-0001-9301-2673
Yaoqiang ShiFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.ORCID http://orcid.org/0000-0001-6076-7711
A-Mei ZhangFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.ORCID http://orcid.org/0000-0002-6681-3376
Xueshan XiaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.ORCID http://orcid.org/0000-0003-1120-8870
Yue FangFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.ORCID http://orcid.org/0000-0002-5280-2043
Xiaoqin MaoMolecular Medicine Center of Yunnan Province, Kunming, Yunnan, China.ORCID http://orcid.org/0000-0002-3022-9063
Jie JiangMolecular Medicine Center of Yunnan Province, Kunming, Yunnan, China.ORCID http://orcid.org/0000-0003-2695-4147
Yuzhu SongFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.ORCID http://orcid.org/0000-0002-9836-0252
Guangying YangFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.ORCID http://orcid.org/0000-0002-4058-9863

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, the rapid and accurate detection of K. pneumoniae is important for the timely treatment of infectious patients. This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse blood with K. pneumoniae were used for sensitivity analysis. The detection limit of the LAMP was 1 bacterium/reaction. The results showed that the LAMP targeted to ureR_1 is a fast, specific, sensitive, inexpensive, and suitable method for the detection of K. pneumoniae.

Indexed as

Genes, BacterialDNA PrimersKlebsiella pneumoniaeLimit of DetectionNucleic Acid Amplification TechniquesPlasmidsPolymerase Chain ReactionReproducibility of ResultsSequence Analysis, DNATemperatureTime FactorsDNA Primers

Identifiers

PMID30916220
PMCPMC6437934

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.