ArticleApplied microbiology and biotechnology2019
Identification of genes involved in shea butter biosynthesis from Vitellaria paradoxa fruits through transcriptomics and functional heterologous expression.
Article in Applied microbiology and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 28 citations in OpenAlex.
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- Genome-wide association study of fat content and fatty acid composition of shea tree (Vitellaria paradoxa C.F. Gaertn subsp. paradoxa).BMC genomics · 2025Article
- Advancing Insights into Probiotics during Vegetable Fermentation.Foods (Basel, Switzerland) · 2023Review
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- Insight into maize gene expression profiles responses to symbiotic bacteria derived from Helicoverpa armigera and Ostrinia furnacalis.Archives of microbiology · 2021Article
- Yeast Synthetic Biology for the Production ofMolecules (Basel, Switzerland) · 2021Review
- Developments in Fatty Acid-Derived Insect Pheromone Production Using Engineered Yeasts.Frontiers in microbiology · 2021Review
- Exploring Gut Microbiota in Patients with Colorectal Disease Based on 16S rRNA Gene Amplicon and Shallow Metagenomic Sequencing.Frontiers in molecular biosciences · 2021Article
- Genomic Resources to Guide Improvement of the Shea Tree.Frontiers in plant science · 2021Article
- Advances in Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2020Review
- Metabolic Engineering for Glycyrrhetinic Acid Production inFrontiers in bioengineering and biotechnology · 2020Review
- Recent Advances on Feasible Strategies for Monoterpenoid Production inFrontiers in bioengineering and biotechnology · 2020Review
- The complete chloroplast genome sequence ofMitochondrial DNA. Part B, Resources · 2019Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Shea tree (Vitellaria paradoxa) is one economically important plant species that mainly distributes in West Africa. Shea butter extracted from shea fruit kernels can be used as valuable products in the food and cosmetic industries. The most valuable composition in shea butter was one kind of triacylglycerol (TAG), 1,3-distearoyl-2-oleoyl-glycerol (SOS, C18:0-C18:1-C18:0). However, shea butter production is limited and little is known about the genetic information of shea tree. In this study, we tried to reveal genetic information of shea tree and identified shea TAG biosynthetic genes for future shea butter production in yeast cell factories. First, we measured lipid content, lipid composition, and TAG composition of seven shea fruits at different ripe stages. Then, we performed transcriptome analysis on two shea fruits containing obviously different levels of SOS and revealed a list of TAG biosynthetic genes potentially involved in TAG biosynthesis. In total, 4 glycerol-3-phosphate acyltransferase (GPAT) genes, 8 lysophospholipid acyltransferase (LPAT) genes, and 11 diacylglycerol acyltransferase (DGAT) genes in TAG biosynthetic pathway were predicted from the assembled transcriptome and 14 of them were cloned from shea fruit cDNA. Furthermore, the heterologous expression of these 14 potential GPAT, LPAT, and DGAT genes in Saccharomyces cerevisiae changed yeast fatty acid and lipid profiles, suggesting that they functioned in S. cerevisiae. Moreover, two shea DGAT genes, VpDGAT1 and VpDGAT7, were identified as functional DGATs in shea tree, showing they might be useful for shea butter (SOS) production in yeast cell factories.
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