ArticleScience advances2019
Single-molecule polarization microscopy of DNA intercalators sheds light on the structure of S-DNA.
Article in Science advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- From single DNA molecule to nanoparticle formation: mechanistic basis for monocationic aromatic drug-induced DNA frameworks.Journal of nanobiotechnology · 2026Article
- Single molecule orientation and localization microscopy.Nature photonics · 2025Article
- Volumetric imaging of the 3D orientation of cellular structures with a polarized fluorescence light-sheet microscope.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Design and Fabrication of Ultrathin Metallic Phase Shifters for Visible and Near-Infrared Wavelengths.Micromachines · 2025Article
- Force-induced melting and S-DNA pathways for DNA overstretching exhibit distinct kinetics.Nucleic acids research · 2025Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- Force-enhanced sensitive and specific detection of DNA-intercalative agents directly from microorganisms at single-molecule level.Nucleic acids research · 2024Article
- POLCAM: instant molecular orientation microscopy for the life sciences.Nature methods · 2024Article
- Unfolding rates of 1:1 and 2:1 complex of CX-5461 and c-Biophysics reports · 2024Article
- Single-Molecule Orientation Imaging Reveals the Nano-Architecture of Amyloid Fibrils Undergoing Growth and Decay.Nano letters · 2024Article
- Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA.Nature communications · 2024Article
- Single-Molecule Orientation Imaging Reveals the Nano-Architecture of Amyloid Fibrils Undergoing Growth and Decay.bioRxiv : the preprint server for biology · 2024Article
- Three-dimensional spatio-angular fluorescence microscopy with a polarized dual-view inverted selective-plane illumination microscope (pol-diSPIM).bioRxiv : the preprint server for biology · 2024Article
- Article
- DNA as grabbers and steerers of quantum emitters.Nanophotonics (Berlin, Germany) · 2023Review
- Six-Dimensional Single-Molecule Imaging with Isotropic Resolution using a Multi-View Reflector Microscope.Nature photonics · 2023Article
- Fluorescence polarization modulation super-resolution imaging provides refined dynamics orientation processes in biological samples.Light, science & applications · 2022Article
- Photon efficient orientation estimation using polarization modulation in single-molecule localization microscopy.Biomedical optics express · 2022Article
- Resolving the Three-Dimensional Rotational and Translational Dynamics of Single Molecules Using Radially and Azimuthally Polarized Fluorescence.Nano letters · 2022Article
- Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble Using a Polarized Vortex Point Spread Function.The journal of physical chemistry. B · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA structural transitions facilitate genomic processes, mediate drug-DNA interactions, and inform the development of emerging DNA-based biotechnology such as programmable materials and DNA origami. While some features of DNA conformational changes are well characterized, fundamental information such as the orientations of the DNA base pairs is unknown. Here, we use concurrent fluorescence polarization imaging and DNA manipulation experiments to probe the structure of S-DNA, an elusive, elongated conformation that can be accessed by mechanical overstretching. To this end, we directly quantify the orientations and rotational dynamics of fluorescent DNA-intercalated dyes. At extensions beyond the DNA overstretching transition, intercalators adopt a tilted (θ ~ 54°) orientation relative to the DNA axis, distinct from the nearly perpendicular orientation (θ ~ 90°) normally assumed at lower extensions. These results provide the first experimental evidence that S-DNA has substantially inclined base pairs relative to those of the standard (Watson-Crick) B-DNA conformation.
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